Journal ArticleDOI
Correlation of glyoxalase I activity with cell proliferation inDatura callus culture
TLDR
With the addition of mitotic inhibitors, vinblastine and methylglyoxal in the growth medium, the enzyme activity was inhibited by 92 percent and 50 percent respectively, at the most effective concentration and the callus growth was also reduced.Abstract:
Glyoxalase-I activity in growingDatura callus showed 184% increase with the age of the culture. Spermidine increased the enzyme activity together with DNA and protein synthesis. With the addition of mitotic inhibitors, vinblastine and methylglyoxal in the growth medium, the enzyme activity was inhibited by 92 percent and 50 percent respectively, at the most effective concentration and the callus growth was also reduced. Similar results were obtained with specific glyoxalase I inhibitors, iso-ascorbate and squaric acid.read more
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Glyoxalase I from Brassica juncea: molecular cloning, regulation and its over-expression confer tolerance in transgenic tobacco under stress.
TL;DR: An important role of glyoxalase I is suggested in conferring tolerance to plants under stress conditions by showing that the tolerance to different salt concentrations was correlated with the degree of Gly I expression.
Journal Article
Stress-induced changes of methylglyoxal level and glyoxalase I activity in pumpkin seedlings and cDNA cloning of glyoxalase I gene.
TL;DR: The stress-induced increases in methylglyoxal level, glyoxalase I activity and Gly I transcript found in the present study suggest an important role of glyoxAlase I in conferring tolerance to plants under stress conditions and show that the gly oxalase pathway is the main detoxification pathway of methylgly oxal in plants.
Journal ArticleDOI
Genome-wide analysis of rice and Arabidopsis identifies two glyoxalase genes that are highly expressed in abiotic stresses
TL;DR: It is shown that overproduction of GLY I and/or GLY II enzymes in transgenic plants provide tolerance towards salinity and heavy metal stresses and qRT-PCR is performed in two contrasting rice genotypes, i.e., IR64 and Pokkali where OsGLyI6 and OsGLYI11 are found to be highly stress inducible.
Journal ArticleDOI
An overview on the role of methylglyoxal and glyoxalases in plants.
TL;DR: Overexpression of the glyoxalase pathway in transgenic tobacco and rice plants has been found to check an increase of ROS and MG under stress conditions by maintaining glutathione homeostasis and antioxidant enzyme levels.
Journal ArticleDOI
Studies on glyphosate-induced carcinogenicity in mouse skin: A proteomic approach
TL;DR: The results suggested that glyphosate has tumor promoting potential in skin carcinogenesis and its mechanism seems to be similar to TPA.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Book ChapterDOI
[99] Determination of nucleic acids in tissues by pentose analysis
TL;DR: The extraction methods described were developed for nucleic acid determinations by spectrophotometric methods and it has become quite clear that the separations are not sufficiently refined for isotopic work.
Journal ArticleDOI
1,4-Diaminobutane (Putrescine), Spermidine, and Spermine
C W Tabor,H Tabor +1 more
TL;DR: No specific mechanism has been firmly established for the action of the polyamines in vivo, however, it is clear that these compounds are physiologically important, and further work is necessary to establish the mechanism of their action.