Cortical Excitatory Neurons and Glia, But Not GABAergic Neurons, Are Produced in the Emx1-Expressing Lineage
Jessica A. Gorski,Tiffany Talley,Mengsheng Qiu,Luis Puelles,John L.R. Rubenstein,Kevin R. Jones +5 more
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TLDR
A strain of mice is generated that expresses the Cre recombinase in a spatial and temporal pattern like that observed for Emx1, and it is demonstrated that radial glia, Cajal-Retzius cells, glutamatergic neurons, astrocytes, and oligodendrocytic cells of most pallial structures originate from an EmX1-expressing lineage.Abstract:
By homologous recombination of an internal ribosome entry site and Cre recombinase coding region into the 3′-untranslated region of the mouse Emx1 gene, we have generated a strain of mice, Emx1IREScre, that expresses the Cre recombinase in a spatial and temporal pattern like that observed for Emx1. When mated to reporter strains, these mice are a sensitive means to fate-map the Emx1-expressing cells of the developing forebrain. Our results demonstrate that radial glia, Cajal-Retzius cells, glutamatergic neurons, astrocytes, and oligodendrocytes of most pallial structures originate from an Emx1-expressing lineage. On the other hand, most of the pallial GABAergic neurons arise outside the Emx1-expressing lineage. Structures that are located near the basal ganglia (e.g., the amygdala and endopiriform nuclei) are not uniformly derived from Emx1-expressing cells.read more
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A robust and high-throughput Cre reporting and characterization system for the whole mouse brain
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The glial nature of embryonic and adult neural stem cells
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Neuronal subtype specification in the cerebral cortex.
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Competing waves of oligodendrocytes in the forebrain and postnatal elimination of an embryonic lineage.
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Oscar Marín,John L.R. Rubenstein +1 more
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