Journal ArticleDOI
Covalent attachment of chelating groups to macromolecules.
TLDR
A simple procedure for covalently coupling diethylenetriaminepentaacetic acid to proteins and creates hexadentate chelating sites for metal ions on proteins is described.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1977-07-25. It has received 379 citations till now. The article focuses on the topics: Macromolecule.read more
Citations
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Journal ArticleDOI
Radioactive labeling of antibody: a simple and efficient method
Donald Hnatowich,W.W. Layne,R.L. Childs,D. Lanteigne,MA Davis,Thomas W. Griffin,Paul W. Doherty +6 more
TL;DR: A simple and efficient method of covalently coupling the strong chelator diethylenetriaminepentaacetic acid to proteins was developed for radiolabeling immunoglobulin G antibodies.
Journal ArticleDOI
Radiometal-Labeled Agents (Non-Technetium) for Diagnostic Imaging
TL;DR: This review will discuss the non-technetium-labeled radiometal- labeled agents used in gamma scintigraphy and positron emission tomography (PET) and their uses in nuclear medicine.
Journal ArticleDOI
Conjugation of antibodies with bifunctional chelating agents: Isothiocyanate and bromoacetamide reagents, methods of analysis, and subsequent addition of metal ions☆
Claude F. Meares,Michael J. McCall,Dayton T. Reardan,David A. Goodwin,David A. Goodwin,Carol I. Diamanti,Carol I. Diamanti,Maureen McTigue,Maureen McTigue +8 more
TL;DR: The labeled products retain their immunoreactivity, as illustrated by experiments in vivo with chelate-conjugated antibody to mouse I-AK antigen.
Journal ArticleDOI
The preparation and labeling of DTPA-coupled albumin.
TL;DR: The simplicity with which albumin has been coupled with DTPA by this method contrasts sharply with existing methods and is an attractive area of research for the labeling of a variety of proteins with a varietyof metallic radionuclides.
Journal ArticleDOI
Metal complexes as diagnostic tools
TL;DR: This review summarizes some of the developments of metal complexes and metal-complex-bioconjugates for the diagnosis of disease states that have occurred over the past 10 years, and demonstrates the significant progress made in the field of coordination chemistry as it applies to the development of diagnostic imaging agents.
References
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Journal ArticleDOI
Protein iodination with solid state lactoperoxidase.
Gary S. David,Ralph A. Reisfeld +1 more
Journal ArticleDOI
Selective binding of metal ions to macromolecules using bifunctional analogs of EDTA
Journal ArticleDOI
Chelating agents for the binding of metal ions to macromolecules.
TL;DR: The preparation of chelating agents whose complexes can interact, in some selected manner, with biological macromolecules could make possible several new applications of metal ions as probes of biological systems.
Journal ArticleDOI
Attachment of Metal-chelating Functional Groups to Hen Egg White Lysozyme AN APPROACH TO INTRODUCING HEAVY ATOMS INTO PROTEIN CRYSTALS
TL;DR: It is shown that the picolinamidine group strongly chelates d orbital transition metal ions and both of the derivatives of lysozyme can be induced to form large crystals under suitable conditions, and both possess considerable lytic activity.
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