Journal ArticleDOI
Cytotoxic Effects of Leukocytes Triggered by Complement Bound to Target Cells
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Chromium-51-labeled chicken erythrocytes treated with rabbit anti-Forssman antibody and the first four or the first seven components of human complement, released isotope upon exposure to human leukocytes, which affected both types of target cell-complement intermediates.Abstract:
Chromium-51-labeled chicken erythrocytes (E), treated with rabbit anti-Forssman antibody (A) and the first four (C1-4) or the first seven (C1-7) components of human complement (C), released isotope upon exposure to human leukocytes. Isotope release from EACJ-7 cells proceeded more rapidly and was more extensive than that from EACI-3 cells. Lysis of these cells was suppressed by pretreatment of leukocytes with antimycinA. Monocyte-enriched leukocyte preparations affected both types of target cell-complement intermediates, whereas purified lymphocytes lysed EACI-7 cells but not EACI-3 cells.read more
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Journal ArticleDOI
A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization.
TL;DR: A population of lymphoid cells from several animal species, including man, was identified through a membrane receptor which binds sheep red blood cells treated with antibody and complement, and these cells could be distinguished by their different abilities to bind EAC, CRL and non-CRL from mouse lymphoid organs.
Book ChapterDOI
Lymphocyte-Mediated Cytotoxicity And Blocking Serum Activity To Tumor Antigens
TL;DR: The studies reviewed in the chapter have given a relatively large amount of evidence for various cell-mediated and humoral immunological reactions against growing tumors, and at least some of the reactions observed in vitro appear to be able to influence tumor growth in vivo.
Book ChapterDOI
Cytotoxic effects of lymphoid cells in vitro.
Peter Perlmann,Göran Holm +1 more
TL;DR: The study of the various pathways by which lymphoid cells can become cytotoxic has been helpful for the understanding of effector role of these cells in cell-destructive reactions in general.
Journal ArticleDOI
THE CELLULAR BASIS OF IMMUNOLOGICAL RESPONSES: A Synthesis of Some Current Views
TL;DR: The cooperative function of T-lymphoblasts would account for the relationship often observed between delayed hypersensitivity and humoral-antibody formation, and for the occurrence of allergen-induced blastcell transformation of lymphocytes from allergic patients with anaphylactic - type hypersensitivity.
Journal ArticleDOI
The complement system of man. I.
Shaun Ruddy,Gigli I,K F Austen +2 more
TL;DR: A large number of these abnormalities are related to Epstein-Barr virus infection, and the use of chemotherapy to correct these problems is a natural progression of disease.
References
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Journal Article
Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs.
TL;DR: The in vitro cytotoxic effect of spleen cells of mice immunized by tumour allografts was studied by measuring target cell inactivation as a function of release of radioactive label (51Cr) or loss of cloning efficiency.
Journal ArticleDOI
Separation of lymphocytes, polymorphonuclear leukocytes and monocytes on glass columns, including tissue culture observations.
Yale Rabinowitz,Yale Rabinowitz +1 more
TL;DR: The viability of column-separated cells was shown by their non-staining with trypan blue, motility, phagocytic ability, oxygen consumption, and survival or development in tissue culture, whereas Nowell’s blast-like, dividing, phytohemagglutinin cells were produced only in cultures containing lymphocytes.
Journal ArticleDOI
Red cells coated with immunoglobulin G: binding and sphering by mononuclear cells in man.
TL;DR: It is inferred that mononuclear cells have specific surface receptors for immunoglobulin G and that these provide a critical phase of the mechanism in vivo, whereby red cells or other particles coated with antibody are apprehended and destroyed.
Journal ArticleDOI
ISOLATION OF ß1F-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT
TL;DR: The hypothesis that C'5 and C'6 form a functional unit was supported by the finding that both components interact with each other in solution resulting in the formation of a complex.
Journal ArticleDOI
The isolation and selected properties of blood monocytes.
TL;DR: In vitro cultivation of homogeneous populations of monocytes results in an increase in cell size, number of mitochondria, and phase-dense granules of the centrosphere, and the phagocytic activity of cultured monocytes may be enhanced by a purified bacterial lipopolysaccharide.