Book ChapterDOI
Cytotoxic effects of lymphoid cells in vitro.
Peter Perlmann,Göran Holm +1 more
TLDR
The study of the various pathways by which lymphoid cells can become cytotoxic has been helpful for the understanding of effector role of these cells in cell-destructive reactions in general.Abstract:
Publisher Summary This chapter discusses the cytotoxic effects of lymphoid cells in vitro. The chapter discusses the complex problem of different types of cytotoxic effects of lymphoid cells. These outstanding workers in the field have managed to present a cohesive picture of the various effects on the target cells. The role of “nonspecific” factors is particularly well clarified. The interrelationships among contact lysis, release of pharmacologically active substances, and the terminal components of the complement system are given in the chapter for special consideration. In an in vitro model, it is shown that lymphoid cells from sensitized donors destroy tissue culture cells carrying the antigen to which the cell donor is sensitized. This type of cytolytic reactions is encountered in a great variety of immune situations, comprising all those mentioned in the chapter. The cell that initiates in vitro cytotoxic reaction is assumed to be the sensitized lymphocyte, equipped with its own recognition sites for antigen on the cells that are destroyed. Although this may be true in many situations, it now seems clear that “normal” lymphoid cells can become cytotoxic to other cells by a variety of pathways. The study of the various pathways by which lymphoid cells can become cytotoxic has been helpful for the understanding of effector role of these cells in cell-destructive reactions in general.read more
Citations
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Book ChapterDOI
Biology of natural killer cells.
TL;DR: The existence of NK cells has prompted a reinterpretation of both the studies of specific cytotoxicity against spontaneous human tumors and the theory of immune surveillance, at least in its most restrictive interpretation.
Journal ArticleDOI
Fas and Perforin Pathways as Major Mechanisms of T Cell-Mediated Cytotoxicity
David Kägi,Françoise Vignaux,Birgit Ledermann,Kurt Bürki,Valérie Depraetere,Shigekazu Nagata,Hans Hengartner,Pierre Golstein +7 more
TL;DR: The perforin- and Fas-based mechanisms may account for all T cell-mediated cytotoxicity in short-term in vitro assays, and no third mechanism was detected.
Book ChapterDOI
Cell-Mediated Cytotoxicity, Allograft Rejection, and Tumor Immunity
TL;DR: Specific cell-mediated cytotoxicity in vitro can be divided into three categories according to the nature of the effector cells, which is most often highly specific and requires intimate contact rather than release of diffusible toxic factors.
Book ChapterDOI
The interactions of lectins with animal cell surfaces.
TL;DR: This chapter discusses the interactions of lectins with animal cell surfaces, which have proven to be quite useful for clinical blood typing and structural studies of blood group substances, in analysis of the surface structure of normal and tumor cells, and so on.
References
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Journal ArticleDOI
Demonstration of tumor-specific antigens in human colonic carcinomata by immunological tolerance and absorption techniques
Phil Gold,Samuel O. Freedman +1 more
TL;DR: It was shown that the tumor-specific antibodies were not directed against bacterial contaminants or against the unusually high concentrations of fibrin found in many neoplastic tissues.
Journal ArticleDOI
Immunization of dissociated spleen cell cultures from normal mice
TL;DR: There is an increase in the ability to discriminate between different varieties of homologous erythrocytes and the in vitro response does not appear to be limited by whatever mechanisms regulate the in vivo response.
Journal ArticleDOI
Specific carcinoembryonic antigens of the human digestive system
Phil Gold,Samuel O. Freedman +1 more
TL;DR: It was concluded that the carcinoembryonic antigens represent cellular constituents which are repressed during the course of differentiation of the normal digestive system epithelium and reappear in the corresponding malignant cells by a process of derepressive-dedifferentiation.
Journal ArticleDOI
Mechanism of a Reaction in Vitro Associated with Delayed-Type Hypersensitivity
Barry R. Bloom,Boyce Bennett +1 more
TL;DR: The cell type responsible for inhibition by antigen of migration in vitro of peritoneal exudate cells obtained from tuberculin-hypersensitive guinea pigs was studied and elaborated into the medium a soluble material capable of inhibiting migration of normal exudates.
Journal Article
Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs.
TL;DR: The in vitro cytotoxic effect of spleen cells of mice immunized by tumour allografts was studied by measuring target cell inactivation as a function of release of radioactive label (51Cr) or loss of cloning efficiency.
Related Papers (5)
Cell-Mediated Cytotoxicity, Allograft Rejection, and Tumor Immunity
Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs.
Contactual lysis of antibody-coated chicken erythrocytes by purified lymphocytes☆
Peter Perlmann,Hedvig Perlmann +1 more