Journal ArticleDOI
De novo biosynthesis of 2-phenylethanol in engineered Pichia pastoris
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TLDR
The engineering of Pichia pastoris is demonstrated to produce 2-PE directly from simple sugars for the first time and showed the potential of P. pastoris as a host strain to produce industrially interested 2- PE by metabolic engineering.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2020-02-01. It has received 24 citations till now. The article focuses on the topics: Pichia pastoris & Metabolic engineering.read more
Citations
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Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts.
TL;DR: In this article, the authors provide an insight into the recent advances and challenges of systems and synthetic biology as well as metabolic engineering endeavours towards the commercial usage of non-conventional yeasts.
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Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products.
TL;DR: A review of Pichia pastoris (a.k.a. Komagataella phaffii) as a synthetic biology chassis for the production of value-added natural products in metabolically engineered P. pastoris strains is presented in this paper.
Journal ArticleDOI
Current state of aromatics production using yeast: achievements and challenges
TL;DR: The recent development of de novo biosynthesis of aromatics derived from the shikimate pathway in yeasts, including the model Saccharomyces cerevisiae as well as other non-conventional species are provided.
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Recent Advances in Metabolically Engineered Microorganisms for the Production of Aromatic Chemicals Derived From Aromatic Amino Acids.
TL;DR: This work systematically and comprehensively review the recent advancements in metabolic engineering and synthetic biology in the microbial production of aromatic amino acid derivatives, stilbenes, and benzylisoquinoline alkaloids.
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Metabolic engineering of Pichia pastoris for production of isopentanol (3-Methyl-1-butanol)
TL;DR: The industrially-relevant yeast Pichia pastoris is engineered to produce the platform chemical 3-methyl-1-butanol (3M1B, isopentanol) directly from the renewable carbon source glucose, and overexpressed the endogenous valine and leucine biosynthetic pathways to increase the production of the key pathway intermediate, 2-KIC.
References
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Journal ArticleDOI
Biotechnological production of 2-phenylethanol
TL;DR: This review describes the microbial production of 2-PE, and also summarizes the chemical syntheses and the market situation.
Journal ArticleDOI
Recent advances in biotechnological production of 2-phenylethanol.
Dongliang Hua,Ping Xu +1 more
TL;DR: Recent advances in biotechnological production of 2-PE are reviewed, with emphasis on the strategies used to increase production and the applications of in situ product removal techniques.
Journal ArticleDOI
Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate
Wiparat Siripong,Philipp Wolf,Theodora Puspowangi Kusumoputri,Joe James Downes,Kanokarn Kocharin,Sutipa Tanapongpipat,Weerawat Runguphan +6 more
TL;DR: This work engineered Pichia pastoris, an industrial workhorse in heterologous enzyme production, to produce the biofuel isobutanol from two renewable carbon sources, glucose and glycerol, and exploited the yeast’s amino acid biosynthetic pathway and diverted the amino acid intermediates to the 2-keto acid degradation pathway for higher alcohol production.
Journal ArticleDOI
Production of the sesquiterpenoid (+)-nootkatone by metabolic engineering of Pichia pastoris.
Tamara Wriessnegger,Peter Andreas Augustin,Matthias Engleder,Erich Leitner,Monika Müller,Iwona Kaluzna,Martin Schürmann,Daniel Mink,Günther Zellnig,Helmut Schwab,Harald Pichler +10 more
TL;DR: Intracellular production of (+)-valencene by co-expression of valencene synthase from Callitropsis nootkatensis resolved the phase-transfer issues of (+-valencenes.
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Production of aromatic compounds by metabolically engineered Escherichia coli with an expanded shikimate pathway.
TL;DR: It is shown here that six aromatic compounds are able to be produced from renewable resources without supplementing with expensive precursors and each of the six constructed strains was able to successfully produce a different aromatic compound as a major product.
Related Papers (5)
Metabolic engineering of Saccharomyces cerevisiae for the production of 2-phenylethanol via Ehrlich pathway
Bosu Kim,Bo-Ram Cho,Ji-Sook Hahn +2 more