Journal ArticleDOI
Metabolic engineering of Pichia pastoris for production of isopentanol (3-Methyl-1-butanol)
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TLDR
The industrially-relevant yeast Pichia pastoris is engineered to produce the platform chemical 3-methyl-1-butanol (3M1B, isopentanol) directly from the renewable carbon source glucose, and overexpressed the endogenous valine and leucine biosynthetic pathways to increase the production of the key pathway intermediate, 2-KIC.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2020-08-01. It has received 24 citations till now. The article focuses on the topics: Pichia pastoris & Metabolic engineering.read more
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Novel CRISPR-Cas Systems: An Updated Review of the Current Achievements, Applications, and Future Research Perspectives.
Sweta Nidhi,Uttpal Anand,Patrik Oleksak,Pooja Tripathi,Jonathan A. Lal,George Thomas,Kamil Kuca,Vijay Tripathi +7 more
TL;DR: A comprehensive review of the current state of the CRISPR-Cas revolution can be found in this article, where the authors aim to elucidate the current and future trends related to opportunities and challenges including ethical concerns.
Journal ArticleDOI
Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products.
TL;DR: A review of Pichia pastoris (a.k.a. Komagataella phaffii) as a synthetic biology chassis for the production of value-added natural products in metabolically engineered P. pastoris strains is presented in this paper.
Journal ArticleDOI
Bioengineered microbial platforms for biomass-derived biofuel production - A review.
TL;DR: A review focusing on exploiting metabolically engineered microbes as promising cell factories for the enhanced production of advanced biofuels is presented in this article, where a wide variety of new products with industrial relevance through metabolic pathway engineering or optimizing native metabolic pathways.
Journal ArticleDOI
Bioengineered microbial platforms for biomass-derived biofuel production – A review
TL;DR: A review focusing on exploiting metabolically engineered microbes as promising cell factories for the enhanced production of advanced biofuels is presented in this article , where a wide variety of new products with industrial relevance through metabolic pathway engineering or optimizing native metabolic pathways.
Journal ArticleDOI
Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR/Cas9.
Jucan Gao,Junhao Xu,Yimeng Zuo,Cuifang Ye,Leijie Jiang,Linjuan Feng,Lei Huang,Zhinan Xu,Jiazhang Lian +8 more
TL;DR: A CRISPR-based synthetic biology toolkit is established and can be employed to construct P. pastoris cell factories with high efficiency and enabled the integration of one locus, two loci, and three loci with efficiencies as high as ∼100, ∼93, and ∼75%, respectively, in P. Pastoris GS115 strain.
References
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Journal ArticleDOI
Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
TL;DR: This strategy uses the host’s highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis to achieve high-yield, high-specificity production of isobutanol from glucose.
Journal ArticleDOI
Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
Eric J. Steen,Yisheng Kang,Gregory Bokinsky,Zhihao Hu,Andreas W. Schirmer,Amy McClure,Stephen B. del Cardayre,Jay D. Keasling +7 more
TL;DR: The engineering of Escherichia coli is demonstrated to produce structurally tailored fatty esters (biodiesel), fatty alcohols, and waxes directly from simple sugars, a step towards producing these compounds directly from hemicellulose, a major component of plant-derived biomass.
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Microbial engineering for the production of advanced biofuels
Pamela Peralta-Yahya,Pamela Peralta-Yahya,Fuzhong Zhang,Fuzhong Zhang,Stephen B. del Cardayre,Jay D. Keasling +5 more
TL;DR: Data-driven and synthetic-biology approaches can be used to optimize both the host and pathways to maximize fuel production, and to compete with more conventional fuels.
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Microbial Biosynthesis of Alkanes
TL;DR: The discovery of an alkane biosynthesis pathway in cyanobacteria that converts intermediates of fatty acid metabolism to alkanes and alkenes is described and is likely to be a valuable tool in the production of biofuels.
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Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.
TL;DR: This review refers to established tools in protein expression in P. pastoris and highlights novel developments in the areas of expression vector design, host strain engineering and screening for high-level expression strains.