Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides. Succinimide-linked reactions that contribute to protein degradation.
Terrence L. Geiger,Steven Clarke +1 more
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TLDR
These studies indicate that both aspartic acid and asparagine residues may be hot spots for the nonenzymatic degradation of proteins, especially in cells such as erythrocytes and eye lens, where these macromolecules must function for periods of about 120 days and 80 years, respectively.About:
This article is published in Journal of Biological Chemistry.The article was published on 1987-01-15 and is currently open access. It has received 1252 citations till now. The article focuses on the topics: Isoaspartate & Deamidation.read more
Citations
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Stability of Protein Pharmaceuticals: An Update
TL;DR: This review summarizes the advances that have been made since then regarding protein stabilization and formulation and discusses the current understanding of chemical and physical instability.
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The role of collagen in bone strength.
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Oxidative stress-induced cataract: mechanism of action.
TL;DR: H2O2 is the major oxidant involved in cataract formation and the data suggest that the epithelial cell layer is the initial site of attack by oxidative stress and that involvement of the lens fibers follows, leading to cortical cataracts.
References
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Rapid analysis of amino acids using pre-column derivatization.
TL;DR: The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydrolyzates and the liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids.
Book
Buffers for pH and metal ion control
D. D. Perrin,Boyd Dempsey +1 more
TL;DR: The concept of buffer action and uses of metal-ion buffers, as well as practical Limitations in the Use of Buffers, are explained.
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Conformation of amino acid side-chains in proteins.
Joël Janin,Shoshanna Wodak +1 more
TL;DR: Configurations that are rare for exposed residues are even rarer for buried residues, suggesting that, while the folded structure puts little strain on side-chain conformations, the side- chain positions with the lowest energy in the unfolded structure are chosen preferentially during folding.
Journal ArticleDOI
o-phthaldialdehyde precolumn derivatization and reversed-phase high-performance liquid chromatography of polypeptide hydrolysates and physiological fluids
Barry N. Jones,James P. Gilligan +1 more
TL;DR: Methods for the amino acid analysis of physiological fluids such as serum, urine and cerebrospinal fluid were also developed which employ the above separation procedure for the identification and quantitation of amino acids and other biological amines.
Journal ArticleDOI
Aspartic acid racemisation in the human lens during ageing and in cataract formation
TL;DR: Results are reported here the results of D/L enantiomeric analyses of normal human lenses and cataracts: aspartic acid racemisation was seen during ageing andCataract formation.
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