Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications
Anna Gilpin,Yong Yang +1 more
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TLDR
The focus of this review is to compare the advantages and disadvantages of these methods in terms of their ability to retain desired ECM characteristics for particular tissues and organs.Abstract:
As the gap between donors and patients in need of an organ transplant continues to widen, research in regenerative medicine seeks to provide alternative strategies for treatment. One of the most promising techniques for tissue and organ regeneration is decellularization, in which the extracellular matrix (ECM) is isolated from its native cells and genetic material in order to produce a natural scaffold. The ECM, which ideally retains its inherent structural, biochemical, and biomechanical cues, can then be recellularized to produce a functional tissue or organ. While decellularization can be accomplished using chemical and enzymatic, physical, or combinative methods, each strategy has both benefits and drawbacks. The focus of this review is to compare the advantages and disadvantages of these methods in terms of their ability to retain desired ECM characteristics for particular tissues and organs. Additionally, a few applications of constructs engineered using decellularized cell sheets, tissues, and whole organs are discussed.read more
Citations
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Scaffold fabrication technologies and structure/function properties in bone tissue engineering
TL;DR: A comprehensive review of the current accomplishments on scaffold fabrication techniques, their structure, and function properties for BTE is provided and strategies to improve vascularization potential and immunomodulation are presented.
Journal ArticleDOI
Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds.
Unai Mendibil,Raquel Ruiz-Hernandez,Sugoi Retegi-Carrion,Nerea Garcia-Urquia,Beatriz Olalde-Graells,Ander Abarrategi +5 more
TL;DR: This review provides an overview of the most common methods used to obtain the dECM and summarizes the strategies adopted to decellularize specific tissues, aiming to provide a helpful guide for future research development.
Journal ArticleDOI
Decellularized ECM-derived bioinks: Prospects for the future.
Fatemeh Kabirian,Masoud Mozafari +1 more
TL;DR: This review defines ECM, classifies decellularization agents and techniques, and explains different sources ofECM, and the future perspectives of 3d bioprinting technology are discussed.
Journal ArticleDOI
Decellularized human ovarian scaffold based on a sodium lauryl ester sulfate (SLES)-treated protocol, as a natural three-dimensional scaffold for construction of bioengineered ovaries.
TL;DR: A human ovary-specific scaffold based on a SLES-decellularized protocol as a biomimicry of the natural ovarian niche can be an ideal scaffold used to reconstruct the ovary.
Journal ArticleDOI
Extracellular Matrix-Based Biomaterials and Their Influence Upon Cell Behavior.
TL;DR: Examples of favorable cellular responses of immune and stem cells associated with constructive tissue remodeling of ECM bioscaffolds are described.
References
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Journal ArticleDOI
Hydrogels for tissue engineering: scaffold design variables and applications.
Jeanie L. Drury,David J. Mooney +1 more
TL;DR: Hydrogels are an appealing scaffold material because they are structurally similar to the extracellular matrix of many tissues, can often be processed under relatively mild conditions, and may be delivered in a minimally invasive manner.
Journal ArticleDOI
An overview of tissue and whole organ decellularization processes.
TL;DR: Tissue decellularization with preservation of ECM integrity and bioactivity can be optimized by making educated decisions regarding the agents and techniques utilized during processing.
Journal ArticleDOI
Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Harald C. Ott,Thomas S Matthiesen,Saik Kia Goh,Lauren D. Black,Stefan M. Kren,Theoden I. Netoff,Doris A. Taylor +6 more
TL;DR: Eight constructs decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular architecture, competent a cellular valves and intact chamber geometry that could generate pump function in a modified working heart preparation.
Journal ArticleDOI
The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder
Matthew J. Dalby,Nikolaj Gadegaard,Rahul S. Tare,Abhay Andar,Mathis O. Riehle,Pawel Herzyk,Chris D. W. Wilkinson,Richard O.C. Oreffo +7 more
TL;DR: The use of nanoscale disorder is demonstrated to stimulate human mesenchymal stem cells (MSCs) to produce bone mineral in vitro, in the absence of osteogenic supplements, which has implications for cell therapies.
Journal ArticleDOI
Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments
Adam J. Engler,Maureen A. Griffin,Shamik Sen,Carsten G. Bönnemann,H. Lee Sweeney,Dennis E. Discher +5 more
TL;DR: Contractile myocytes provide a test of the hypothesis that cells sense their mechanical as well as molecular microenvironment, altering expression, organization, and/or morphology accordingly, and have major implications for in vivo introduction of stem cells into diseased or damaged striated muscle of altered mechanical composition.
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