Journal ArticleDOI
Detection of Citrus Huanglongbing by Fourier Transform Infrared—Attenuated Total Reflection Spectroscopy:
Samantha A. Hawkins,Bosoon Park,Gavin H. Poole,Timothy R. Gottwald,William R. Windham,Kurt C. Lawrence +5 more
TLDR
F Fourier transform infrared-attenuated total reflection (FT-IR-ATR) spectroscopy is a candidate for rapid, inexpensive, early detection of the citrus Huanglongbing disease.Abstract:
Citrus Huanglongbing (HLB, also known as citrus greening disease) was discovered in Florida in 2005 and is spreading rapidly amongst the citrus growing regions of the state. Detection via visual symptoms of the disease is not a long-term viable option. New techniques are being developed to test for the disease in its earlier presymptomatic stages. Fourier transform infrared–attenuated total reflection (FT-IR-ATR) spectroscopy is a candidate for rapid, inexpensive, early detection of the disease. The mid-infrared region of the spectrum reveals dramatic changes that take place in the infected leaves when compared to healthy non-infected leaves. The carbohydrates that give rise to peaks in the 900–1180 cm−1 range are reliable in distinguishing leaves from infected plants versus non-infected plants. A model based on chemometrics was developed using the spectra from 179 plants of known disease status. This model then correctly predicted the status of >95% of the plants tested.read more
Citations
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Journal ArticleDOI
Mid-infrared spectroscopy for detection of Huanglongbing (greening) in citrus leaves
TL;DR: This study demonstrates the applicability of mid-infrared spectroscopy for HLB detection in citrus by finding the spectral peak in the region of 9.5 μm was found to be distinctly different between the healthy and HLB-infected leaf samples.
Journal ArticleDOI
Advanced spectroscopic techniques for plant disease diagnostics. A review
TL;DR: In this article, a review discusses recent progress in development of reflectance, infrared, Raman and surface-enhanced Raman spectroscopy for detection and identification of plant diseases.
Journal ArticleDOI
Spectral difference analysis and airborne imaging classification for citrus greening infected trees
TL;DR: In this paper, the authors used ground truthing including field and indoor spectral measurement, infection status along with GPS coordinates for both healthy and infected trees to detect citrus greening infected trees.
Journal ArticleDOI
Early detection of diseases in plant tissue using spectroscopy – applications and limitations
Alfadhl Yahya Khaled,Samsuzana Abd Aziz,Siti Khairunniza Bejo,Nazmi Mat Nawi,Idris Abu Seman,Daniel I. Onwude +5 more
TL;DR: The applications and limitations of spectroscopy techniques (visible/infrared, electrical impedance and fluorescence spectroscope) in early detection of plant disease are reviewed, with particular emphasis given to different spectral level, challenges and future outlook.
Book
Leaf Optical Properties
TL;DR: This book reviews the state-of-the-art research on leaf optics, providing the necessary foundations in physics, chemistry and biology to make the context accessible to readers from various subject backgrounds.
References
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Journal ArticleDOI
Huanglongbing: a destructive, newly-emerging, century-old disease of citrus.
TL;DR: Control of huanglongbing (HLB), a destructive disease of citrus that represents a major threat to the world citrus industry, is preventive and largely based on inoculum elimination by removal of infected trees and chemical treatments against vectors.
Journal ArticleDOI
Real-time PCR in virology
TL;DR: The background, advantages and limitations of real-time PCR are described, the literature as it applies to virus detection in the routine and research laboratory is reviewed and the technology discussed has been applied to other areas of microbiology as well as studies of gene expression and genetic disease.
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Allelic discrimination by nick-translation PCR with fluorogenic probes.
TL;DR: Nick-translation PCR was performed with fluorogenic probes that generated fluorescence from its indicator dye only when the sequence between the indicator and quencher dyes was perfectly complementary to target.
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Asian citrus psyllids (sternorrhyncha: psyllidae) and greening disease of citrus: a literature review and assessment of risk in florida
Susan E. Halbert,K. L. Manjunath +1 more
TL;DR: Management of citrus greening disease is difficult and requires an integrated approach including use of clean stock, elimination of inoculum via voluntary and regulatory means, use of pesticides to control psyllid vectors in the citrus crop, and biological control of psyllID vectors in non-crop reservoirs.
Journal ArticleDOI
Short-range structure in (partially) crystalline potato starch determined with attenuated total reflectance Fourier-transform IR spectroscopy
TL;DR: In this paper, a fast and direct method, based on infrared spectroscopy, for quantitative determination of starch short-range structure has been developed, which can quantify the IR data in terms of shortrange order (SIR) over a range of 10−50% water.