Journal ArticleDOI
Detection of IgE autoantibodies to BP180 and BP230 and their relationship to clinical features in bullous pemphigoid
Takashi Hashimoto,Ayaka Ohzono,Kwesi Teye,Sanae Numata,Sho Hiroyasu,Daisuke Tsuruta,Takahisa Hachiya,K Kuroda,Masahiro Hashiguchi,Tamihiro Kawakami,Norito Ishii +10 more
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TLDR
IgE autoantibodies are considered to be involved in the pathogenesis of bullous pemphigoid (BP), particularly inflammatory and erythematous phenotypes.Abstract:
SummaryBackground
IgE autoantibodies are considered to be involved in the pathogenesis of bullous pemphigoid (BP), particularly inflammatory and erythematous phenotypes.
Objectives
To develop reliable enzyme-linked immunosorbent assays (ELISAs) for the detection of IgE autoantibodies to both BP180 and BP230 in BP sera, and to compare the ELISA results with clinical features.
Methods
We used commercially available IgG ELISAs to develop IgE ELISAs for both BP180 and BP230. To determine the influence of excess amounts of IgG autoantibodies, all normal and BP sera were tested before and after IgG adsorption. The results of the IgE ELISAs were statistically compared with various ELISAs and various clinical parameters, including our own severity scores and BP phenotypes.
Results
IgG adsorption generally showed no changes in sensitivity and specificity for IgE ELISAs, although slight cross-reactivity of anti-IgE secondary antibody to IgG and interference of excess amounts of IgG autoantibodies to IgE reactivity were suggested. IgE autoantibodies to BP180 were found in 21 of 36 BP sera and IgE autoantibodies to BP230 were found in 18 of 36 BP sera. The results of IgG and IgE ELISAs for both BP180 and BP230 were well correlated. IgG and IgE anti-BP180 antibodies correlated with disease activity but IgG and IgE anti-BP230 autoantibodies did not. IgE anti-BP230 autoantibodies correlated with nodular phenotype but not erythematous phenotype.
Conclusions
The results of this study indicated that IgE autoantibodies to both BP180 and BP230 are frequently detected in BP sera. IgE anti-BP180 autoantibodies seemed to be pathogenic, while an association between IgE autoantibodies and inflammatory BP phenotype was not indicated.read more
Citations
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Journal ArticleDOI
Autoimmune Subepidermal Bullous Diseases of the Skin and Mucosae: Clinical Features, Diagnosis, and Management.
TL;DR: The clinical and immunopathological features as well as the pathophysiology of this group of organ-specific autoimmune diseases are reviewed, which involves a number of different autoantigens in the basement membrane zone, are reviewed.
Journal ArticleDOI
New Insights Into the Pathogenesis of Bullous Pemphigoid: 2019 Update.
Giovanni Genovese,Giovanni Genovese,Giovanni Di Zenzo,Emanuele Cozzani,Emilio Berti,Emilio Berti,Massimo Cugno,Massimo Cugno,Angelo V. Marzano,Angelo V. Marzano +9 more
TL;DR: The current concepts and controversies on the complex pathogenesis of BP are reviewed, shedding light on the most recent theories emerging from the literature.
Journal ArticleDOI
Diagnosis of Autoimmune Blistering Diseases
TL;DR: Clinical and immunopathological features of AIBD are presented for the state-of-the art diagnosis of these disorders for both treatment and prognosis.
Journal ArticleDOI
The Role of Eosinophils in Bullous Pemphigoid: A Developing Model of Eosinophil Pathogenicity in Mucocutaneous Disease.
TL;DR: The role of eosinophils in BP is reviewed and a framework for understanding eOSinophil pathogenic mechanisms in mucocutaneous disease is provided.
References
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Journal ArticleDOI
A passive transfer model of the organ-specific autoimmune disease, bullous pemphigoid, using antibodies generated against the hemidesmosomal antigen, BP180.
Zhi Liu,Luis A. Diaz,James L. Troy,James L. Troy,Ann F. Taylor,Daryl J. Emery,Janet A. Fairley,Janet A. Fairley,George J. Giudice +8 more
TL;DR: Subepidermal blistering associated with the human skin diseases bullous pemphigoid and herpes gestationis has been thought to be an IgG autoantibody-mediated process; however, previous attempts to demonstrate the pathogenicity of patientAutoantibodies have been unsuccessful.
Journal Article
Characterization of autoantibodies in pemphigus using antigen-specific enzyme-linked immunosorbent assays with baculovirus-expressed recombinant desmogleins.
Ken Ishii,Masayuki Amagai,Russell P. Hall,Takashi Hashimoto,Atsushi Takayanagi,Shinobu Gamou,Nobuyoshi Shimizu,Takeji Nishikawa +7 more
TL;DR: This ELISA provides a sensitive and highly specific assay for the diagnosis of patients with PV and PF, the correlation of disease activity with serum Ab levels, and a novel tool for investigating the immunopathogenesis of pemphigus.
Journal ArticleDOI
Usefulness of enzyme-linked immunosorbent assay using recombinant desmogleins 1 and 3 for serodiagnosis of pemphigus
Masayuki Amagai,Ayako Komai,Takashi Hashimoto,Yuji Shirakata,Koji Hashimoto,T. Yamada,Yasuo Kitajima,Kazuhiko Ohya,H Iwanami,Takeji Nishikawa +9 more
TL;DR: Dsg1 and Dsg3 ELISAs provide a simple, sensitive and highly specific assay for the diagnosis of patients with PV and PF and that these ELIS as may be a valuable tool to monitor the disease activity.
Journal ArticleDOI
Tight Clustering of Extracellular BP180 Epitopes Recognized by Bullous Pemphigoid Autoantibodies
Detlef Zillikens,Pamela A. Rose,Shawn D. Balding,Zhi Liu,Monica Olague-Marchan,Luis A. Diaz,Luis A. Diaz,George J. Giudice +7 more
TL;DR: Immunoblot analysis of bullous pemphigoid sera immunoadsorbed with a series of recombinant NC16A peptides revealed the presence of three novel autoantigenic sites that, along with the MCW-1 epitope, are clustered within the N-terminal 45 amino acid stretch ofNC16A.
Journal ArticleDOI
Definitions and outcome measures for bullous pemphigoid: Recommendations by an international panel of experts
Dedee F. Murrell,Benjamin S. Daniel,Pascal Joly,Luca Borradori,Masayuki Amagai,Takashi Hashimoto,Frédéric Caux,Branka Marinović,Animesh A. Sinha,Michael Hertl,Philippe Bernard,David A. Sirois,Giuseppe Cianchini,Janet A. Fairley,Marcel F. Jonkman,Amit G. Pandya,David S. Rubenstein,Detlef Zillikens,Aimee S. Payne,David T. Woodley,Giovanna Zambruno,Valeria Aoki,Carlo Pincelli,Luis A. Diaz,Russell P. Hall,Michael Meurer,José M. Mascaró,Enno Schmidt,Hiroshi Shimizu,John J. Zone,Robert A. Swerlick,Daniel Mimouni,Donna A. Culton,Jasna Lipozenčić,Benjamin B. Bince,Sergei A. Grando,Jean-Claude Bystryn,Victoria P. Werth,Victoria P. Werth +38 more
TL;DR: Recommendations from the International Pemphigoid Committee represent 2 years of collaborative efforts to attain mutually acceptable common definitions for BP and proposes a disease extent score, the BP Disease Area Index, to assist in the development of consistent reporting of outcomes.