Journal ArticleDOI
Determination of drug metabolizing enzymes in needle biopsies of human liver.
Reads0
Chats0
TLDR
A micromethod is described for determination of the cytochrome P-450 content, demethylation rate of aminopyrine and p-nitroanisole, the activities of NADPH-cytochrome-c-reductase, pseudocholinesterase and glucose-6-phosphatase in homogenates of the small amount of human liver obtained by needle biopsy.Abstract:
A micromethod is described for determination of the cytochrome P-450 content, demethylation rate of aminopyrine and p-nitroanisole, the activities of NADPH-cytochrome-c-reductase, pseudocholinesterase and glucose-6-phosphatase in homogenates of the small amount of human liver (about 20 mg) obtained by needle biopsy. 1 g of human liver contains 32–38 mg microsomal protein, much less than that present in rat liver (50–60 mg/g liver). The cytochrome P-450 content in normals was 10.8±2.6 nmoles/g liver wet weight (mean±1 S.D.). In severe hepatitis and cirrhosis the cytochrome P-450 content fell to 50% of the control value, whereas mild and moderate hepatitis did not produce any change. Similarly, only in seriously damaged livers were the demethylationrates of both substrates lowered. The activity of the NADPH-cytochrome-c-reductase was 1.88±0.51 micromoles/g liver × min and was not impaired even by severe liver damage. The activities of glucose-6-phosphatase and pseudocholinesterase also fell only in severe liver disease.read more
Citations
More filters
Journal ArticleDOI
The effects of age and liver disease on the disposition and elimination of diazepam in adult man.
TL;DR: The constancy of diazepam clearance indicates that drug plasma concentrations will not accumulate any more in the old than the young, and chronic dosage modifications based on pharmacokinetic considerations are unnecessary.
Journal ArticleDOI
Quantitative determination of cytochrome P-450 in rat liver homogenate
TL;DR: The content of cytochrome P -450 in normal rat liver was estimated to be 50 nmol/g wet weight of liver, and increased significantly after pretreatment of the animals with either phenobarbital or 3-methylcholanthrene.
Journal ArticleDOI
Ontogeny of Hepatic and Renal Systemic Clearance Pathways in Infants Part I
Jane Alcorn,Patrick J. McNamara +1 more
TL;DR: The summary of the current understanding of the ontogeny of individual pathways of hepatic and renal elimination presented in this review should serve as a basis for the continued accruement of age-specific information concerning the ontogenicy of clearance mechanisms in infants.
Journal ArticleDOI
Human cytochromes P450
Julia A. Hasler,Ronald W. Estabrook,Michael Murray,Irina A. Pikuleva,Michael R. Waterman,Jorge H. Capdevila,Vijakumar Holla,Christian F. Helvig,John R. Falck,Geoffrey C. Farrell,Laurence S. Kaminsky,Simon D. Spivack,Eric Boitier,Philippe Beaune +13 more
TL;DR: This review deals with aspects of cytochrome P450s of relevance to human physiology, biochemistry, pharmacology and medicine, including their role in metabolism of endogenous compounds such as steroids and eicosanoids, and the effect of disease on CYP function, CYPs and cancer.
Journal ArticleDOI
Acetaminophen hepatotoxicity: The first 35 years
TL;DR: The lack of enhancement of acetaminophen toxicity by phenytoin and in fact, the potential for reduction of toxicity with that agent is a good example of the evolution of knowledge.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more
TL;DR: The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.
Journal ArticleDOI
The colorimetric estimation of formaldehyde by means of the Hantzsch reaction
TL;DR: A chronology of key events leading up to and including the birth of Bonnichsen, R. K. & Bonner, J. (1952).
Journal ArticleDOI
Microsomal triphosphopyridine nucleotide-cytochrome c reductase of liver.
Charles Williams,Henry Kamin +1 more
TL;DR: The biological role of reduced triphosphopyridine nucleotide (TPNH) and the metabolic pathways of its hydrogen atom and electron appear to be fundamentally different from those of reduced diphosphipyridineucleotide (DPNH).
Related Papers (5)
The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more