Journal ArticleDOI
The microestimation of succinate and the extinction coefficient of cytochrome c.
Reads0
Chats0
About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1959-01-01. It has received 727 citations till now. The article focuses on the topics: Cytochrome c.read more
Citations
More filters
Journal ArticleDOI
Superoxide Dismutase AN ENZYMIC FUNCTION FOR ERYTHROCUPREIN (HEMOCUPREIN)
Joe M. McCord,Irwin Fridovich +1 more
TL;DR: The demonstration that O2·- can reduce ferricytochrome c and tetranitromethane, and that superoxide dismutase, by competing for the superoxide radicals, can markedly inhibit these reactions, is demonstrated.
Journal ArticleDOI
Chemiosmotic coupling in oxidative and photosynthetic phosphorylation
TL;DR: The end result of the coupling between the flows through the o/r and h/d pathways in oxidative phosphorylation in mitochondria is that, for the equivalent of each pair of electrons traversing the respiratory chain, up to 3 anhydro-bond equivalents may normally traverse the h/D pathway from adenosine diphosphate plus inorganic phosphate (ADP +Pi) to water.
Journal ArticleDOI
Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions
Wayne F. Beyer,Irwin Fridovich +1 more
TL;DR: The effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium are investigated to help investigators to avoid the numerous potential pitfalls which necessarily surround these assay methods.
Journal ArticleDOI
The Reduction of Cytochrome c by Milk Xanthine Oxidase
Joe M. McCord,Irwin Fridovich +1 more
TL;DR: The data are consistent with the conclusion that xanthine oxidase generates an unstable reduced form of oxygen, presumably the superoxide anion, and that this radical is the agent which directly reduces cytochrome c and initiates the sulfite-oxygen chain reaction.
Journal ArticleDOI
Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader
Edgar Pick,Diane Mizel +1 more
TL;DR: The principal advantages of this techniques are: the ability to perform the assays directly in the culture plates with cells in situ; the small amounts of cells and reagents needed; its sensitivity and reproducibility; the ease with which kinetic experiments can be done; the large number of samples which can be tested in parallel, and especially the speed and convenience offered by the automated reading and printout of absorbance values.
References
More filters
Journal ArticleDOI
Semiquinones, the Intermediate Steps of Reversible Organic Oxidation-Reduction.
Journal ArticleDOI
Studies on succinic dehydrogenase. II. Isolation and properties of the dehydrogenase from beef heart.
TL;DR: The present paper deals with the purification of the dehydrogenase from beef heart mitochondria and surveys some of its salient catalytic properties and protein characteristics.
Related Papers (5)
Superoxide Dismutase AN ENZYMIC FUNCTION FOR ERYTHROCUPREIN (HEMOCUPREIN)
Joe M. McCord,Irwin Fridovich +1 more