Journal ArticleDOI
Determination of ochratoxin A in wheat after clean-up through a DNA aptamer-based solid phase extraction column.
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TLDR
A DNA aptamer with high affinity and specificity to ochratoxin A was conjugated to a coupling gel and used as sorbent for the preparation of solid phase extraction (SPE) columns that could be re-used up to five times without any loss of performance.About:
This article is published in Food Chemistry.The article was published on 2011-08-01. It has received 103 citations till now. The article focuses on the topics: Solid phase extraction & Detection limit.read more
Citations
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Journal ArticleDOI
Modern trends in solid phase extraction: New sorbent media
Justyna Płotka-Wasylka,Justyna Płotka-Wasylka,Natalia Szczepańska,Natalia Szczepańska,Miguel de la Guardia,Miguel de la Guardia,Jacek Namieśnik,Jacek Namieśnik +7 more
TL;DR: A review of the most important features and application of formats and devices employed in solid phase extraction (SPE) can be found in this paper, where special attention was paid on new trapping media proposed in SPE prior the chromatography analysis, based on the use of nanostructured materials.
Journal ArticleDOI
Challenges and Opportunities for Small Molecule Aptamer Development
Maureen McKeague,Maria C. DeRosa +1 more
TL;DR: The technical challenges of aptamer development for small molecule targets, as well as the opportunities that exist for their application in biosensing and chemical biology are highlighted.
Journal ArticleDOI
Miniaturized solid-phase extraction techniques
TL;DR: In this article, a review summarizes the current state of the art and the future prospects for green analytical chemistry with special emphasis on environment-friendly sample-preparation techniques based on the solid phase.
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Analytical methods for determination of mycotoxins: An update (2009–2014)
Nicholas W. Turner,Heli Bramhmbhatt,Monika Szabo-Vezse,Alessandro Poma,R. D. Coker,Sergey A. Piletsky +5 more
TL;DR: This review summarises traditional methods such as chromatographic and immunochemical techniques, as well as newer approaches such as biosensors, and optical techniques which are becoming more prevalent, which are being more prevalent in the field of mycotoxins.
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Screening and Initial Binding Assessment of Fumonisin B1 Aptamers
Maureen McKeague,Charlotte R. Bradley,Annalisa De Girolamo,Angelo Visconti,J. David Miller,Maria C. DeRosa +5 more
TL;DR: Aptamers are single-stranded oligonucleotides selected using Systematic Evolution of Ligands by EXponential enrichment (SELEX) for their ability to bind to targets with high affinity and specificity and shows potential for use in fumonisin biosensors and solid phase extraction columns.
References
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Journal ArticleDOI
Adaptive recognition by nucleic acid aptamers.
Thomas Hermann,Dinshaw J. Patel +1 more
TL;DR: Structures of aptamer complexes reveal the key molecular interactions conferring specificity to the aptamer-ligand association, including the precise stacking of flat moieties, specific hydrogen bonding, and molecular shape complementarity.
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Aptamers: molecular tools for analytical applications
Teresa Mairal,Veli Cengiz Ozalp,Pablo Lozano Sánchez,Mònica Mir,Ioanis Katakis,Ciara K. O'Sullivan +5 more
TL;DR: The evolving use of aptamers in specific analytical applications such as chromatography, ELISA-type assays, biosensors and affinity PCR as well as current avenues of research and future perspectives conclude this review.
Journal ArticleDOI
Determination of ochratoxin a with a DNA aptamer.
TL;DR: An aptamer that binds with high affinity and specificity to ochratoxin A (OTA), a mycotoxin that occurs in wheat and other foodstuffs, and a quantitative detection method for OTA based on the use of this aptamer are described.
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An aptamer-based quartz crystal protein biosensor.
TL;DR: A quartz crystal biosensor designed to detect concentrations and ligand affinity parameters of free unlabeled proteins in real time demonstrates for the first time that an aptamer-based biosensor can specifically and quantitatively detect an analyte in various complex protein mixes.
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An enzyme-linked oligonucleotide assay.
TL;DR: This work describes an enzyme-linked sandwich assay that uses a SELEX-derived oligonucleotide and demonstrates that these oligon nucleotides can be effective and useful analytical reagents.