Book ChapterDOI
Determination of sialic acids
Gerd Reuter,Roland Schauer +1 more
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TLDR
The determination of sialic acids in serum, urine, or tissues or on cells is often used in clinical applications, because it seems to be a valuable marker for certain malignancies.Abstract:
Publisher Summary This chapter describes isolation, purification, and characterization of monomeric sialic acids. There are two basic procedures for liberating sialic acids from glycosidic linkages—enzymatic and acid hydrolysis. In the former case, a variety of sialidases may be used, which differ in specificity for sialic acid linkage or species. Quantitation of sialic acids usually requires purified samples because a number of substances are known to interfere with certain tests. Colorimetric sialic acid quantitation is still one of the most important methods to determine the amount of the sugars in a given sample accurately. Two basically different tests are used—the orcinol/Fe 3+ /HCl or resorcinol/Cu 2+ /HCl assay and the periodic acid/thiobarbituric acid test. The procedures for a microadaptation of the orcinol/Fe 3+ /HCl and the periodic acid/thiobarbituric acid assays are discussed. The determination of sialic acids in serum, urine, or tissues or on cells is often used in clinical applications, because it seems to be a valuable marker for certain malignancies.read more
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Book ChapterDOI
Sialic Acids in Molecular and Cellular Interactions
Sørge Kelm,Roland Schauer +1 more
TL;DR: The aim of this chapter is to summarize the knowledge about Sias in masking, for example, galactose residues, and to review the progress made during the past few years with respect to Sias as recognition determinants in the adhesion of pathogenic viruses, bacteria, and protozoa, and particularly as binding sites for endogenous cellular interaction molecules.
Journal ArticleDOI
Structural investigations of glycoconjugates at high sensitivity.
Yehia Mechref,Milos V. Novotny +1 more
Journal ArticleDOI
A high molecular mass constituent of cranberry juice inhibits helicobacter pylori adhesion to human gastric mucus
TL;DR: A high molecular mass constituent derived from cranberry juice inhibits the sialic acid-specific adhesion of H. pylori to human gastric mucus and to human erythrocytes.
Book ChapterDOI
Biochemistry and Role of Sialic Acids
TL;DR: Research on the structures, metabolism, and molecular biology, as well as on the biological and clinical importance of sialic acids as components of these glycoconjugates, has therefore intensified during the past several years.
References
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Journal ArticleDOI
The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
Journal ArticleDOI
Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method.
TL;DR: A new method for the quantitive determination of sialic acids is described, which is about 50% more sensitive than the orcinol-hydrochloric acid method generally used and considerably lower with the resorcin reagent.
Journal ArticleDOI
The elderberry (Sambucus nigra L.) bark lectin recognizes the Neu5Ac(alpha 2-6)Gal/GalNAc sequence.
Naoto Shibuya,Irwin J. Goldstein,Willem F. Broekaert,Makuta Nsimba-Lubaki,Benjamin Peeters,Willy J. Peumans +5 more
TL;DR: Carbohydrate binding properties of a new plant lectin isolated from elderberry bark were studied using the techniques of quantitative precipitation, hapten inhibition, and equilibrium dialysis, suggesting the presence of a hydrophibic interacting region adjacent to the carbohydrate binding site.
Journal ArticleDOI
Diversity in the sialic acids
TL;DR: This review attempts to briefly summarize current knowledge concerning the occurrence, structure, biochemistry and biological significance of this diversity in the sialic acids.
Journal ArticleDOI
Sialic acids and their role as biological masks
TL;DR: Sialic acids are a group of sugars occurring mainly as components of glycocojugates in higher organisms and the regulation of molecular and cellular recognition is of outstanding importance.
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