Journal ArticleDOI
Determination of xylanase, beta-glucanase, and cellulase activity.
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TLDR
A simple, robust and highly reproducible method for the determination of xylanase, β-glucanase, and cellulase in commercial feed enzyme preparations is described and is independent of enzyme standards.Abstract:
A simple, robust and highly reproducible method for the determination of xylanase, β-glucanase, and cellulase in commercial feed enzyme preparations is described. The method is based on measurement of reducing moieties released by the enzymes from arabinoxylan, β-glucan, or carboxymethylcellulose (CMC) and is independent of enzyme standards.read more
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Journal ArticleDOI
Who is who in litter decomposition? Metaproteomics reveals major microbial players and their biogeochemical functions
Thomas Schneider,Katharina M. Keiblinger,Emanuel Schmid,Katja Sterflinger-Gleixner,Günther Ellersdorfer,Bernd Roschitzki,Andreas Richter,Andreas Richter,Leo Eberl,Sophie Zechmeister-Boltenstern,Kathrin Riedel +10 more
TL;DR: Fungi were found to be the main producers of extracellular hydrolytic enzymes, with no bacterial hydrolases being detected by the metaproteomics approach, providing evidence that the litter nutrient content and the stoichiometry of C:N:P affect the decomposer community structure and activity.
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Yield performances and changes in enzyme activities of Pleurotus spp. (P. ostreatus and P. sajor-caju) cultivated on different agricultural wastes.
Şebnem Kurt,Saadet Büyükalaca +1 more
TL;DR: Grown on substrates containing wheat bran had higher biological efficiencies and total yields as well as higher CMCase and laccase activities.
Journal ArticleDOI
Optimization of multienzyme production by two mixed strains in solid-state fermentation.
TL;DR: F3 and F4 strains of Aspergillus niger were screened from five strains of fungi to produce multienzyme preparations (containing cellulase, hemicellulase, glucoamylase, pectinase, and acidic proteinase) as dietary supplementation to optimize media.
Journal ArticleDOI
pH pre-corrected liquid hot water pretreatment on corn stover with high hemicellulose recovery and low inhibitors formation
TL;DR: An effective pH pre-corrected liquid hot water pretreatment (LHWP) was developed by employing a small amount of NaOH to accelerate the hemicellulose deacetylation and simultaneously pre-Correct the acid hydrolyzate in situ, and showed that the pHPre-correction can control the hydrolyZate pH.
Journal ArticleDOI
Glycoside hydrolase inventory drives plant polysaccharide deconstruction by the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus
TL;DR: The genome of C. saccharolyticus encodes a range of glycoside hydrolases (GHs) that mediate plant biomass deconstruction by this bacterium as discussed by the authors.