Journal ArticleDOI
Development and evaluation of multiplex RT-LAMP assays for rapid and sensitive detection of foot-and-mouth disease virus
Wataru Yamazaki,Valerie Mioulet,Lee Murray,Mikidache Madi,Takeshi Haga,Naoaki Misawa,Yoichiro Horii,Donald P. King +7 more
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TLDR
The most successful of the four multiplex RT-LAMP assays achieved a diagnostic sensitivity and specificity of 98.0% and 98.1%, and did not falsely detect FMDV in known negatives or samples containing swine vesicle disease virus, vesicular stomatitis virus or vesicles exanthema of swine virus.About:
This article is published in Journal of Virological Methods.The article was published on 2013-09-01. It has received 59 citations till now. The article focuses on the topics: Multiplex & Vesicular exanthema of swine virus.read more
Citations
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Journal ArticleDOI
Evaluation of Two Lyophilized Molecular Assays to Rapidly Detect Foot-and-Mouth Disease Virus Directly from Clinical Samples in Field Settings.
Emma L.A. Howson,Emma L.A. Howson,Bryony Armson,Bryony Armson,Mikidache Madi,Christopher J. Kasanga,S. Kandusi,Raphael Sallu,Eunice C. Chepkwony,A. Siddle,P. Martin,J. Wood,V. Mioulet,Donald P. King,Tiziana Lembo,Sarah Cleaveland,Veronica L. Fowler +16 more
TL;DR: Lyophilized rRT‐PCR and RT‐LAMP assays proved to be reliable in their ability to detect FMDV in a range of clinical samples from acutely infected as well as convalescent cattle, and support the use of highly sensitive molecular assays into field settings for simple and rapid detection of F MDV.
Journal ArticleDOI
A Mismatch-Tolerant Reverse Transcription Loop-Mediated Isothermal Amplification Method and Its Application on Simultaneous Detection of All Four Serotype of Dengue Viruses.
Yi Zhou,Zhenzhou Wan,Shuting Yang,Yingxue Li,Min Li,Binghui Wang,Yihong Hu,Xueshan Xia,Xia Jin,Na Yu,Chiyu Zhang +10 more
TL;DR: A novel mismatch-tolerant LAMP assay and its application in the detection of dengue viruses (DENV), which has a markedly improved amplification efficiency especially for the mutants forming mismatches with internal primers (FIP/BIP) and loop primer (FLP/BLP).
Journal ArticleDOI
Challenges and prospects for the control of foot-and-mouth disease: an African perspective
Francois Frederick Maree,Christopher J. Kasanga,Katherine Anne Scott,Pamela Anne Opperman,Chitray Melanie,Abraham Sangula,Sallu Raphael,Sinkala Yona,Philemon N. Wambura,Donald P. King,David J. Paton,Mark M. Rweyemamu +11 more
TL;DR: Progress that has been achieved in the development of new technologies, including research towards improved tailored vaccines, appropriate vaccine strain selection, vaccine potency, and diagnostics are emphasized, and how it relates to the conditions in Africa is focused on.
Journal ArticleDOI
Field‐Deployable Reverse Transcription‐Insulated Isothermal PCR (RT‐iiPCR) Assay for Rapid and Sensitive Detection of Foot‐and‐Mouth Disease Virus
Aruna Ambagala,Mathew Fisher,M. Goolia,C. Nfon,Tara Furukawa-Stoffer,R. Ortega Polo,Oliver Lung +6 more
TL;DR: In this paper, a reverse transcription-insulated isothermal PCR (RT-iiPCR) was used to detect FMDV RNA in vesicular fluid samples without nucleic acid extraction.
Journal ArticleDOI
Challenges and Economic Implications in the Control of Foot and Mouth Disease in Sub-Saharan Africa: Lessons from the Zambian Experience
Yona Sinkala,Martin Simuunza,Dirk U. Pfeiffer,Hetron Mweemba Munang'andu,Misheck Mulumba,Christopher J. Kasanga,John Bwalya Muma,Aaron S. Mweene +7 more
TL;DR: A review of the control challenges and economic impact of FMD outbreaks in Zambia was made and identified gaps may be considered in the annual appraisal of the FMD national control strategy in order to advance on the progressive control pathway.
References
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Journal ArticleDOI
Loop-mediated isothermal amplification of DNA
Tsugunori Notomi,Hiroto Okayama,Harumi Otawara-shi Masubuchi,Toshihiro Yonekawa,Keiko Watanabe,Nobuyuki Amino,Tetsu Hase +6 more
TL;DR: A novel method that amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions that employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA.
Journal ArticleDOI
Accelerated reaction by loop-mediated isothermal amplification using loop primers.
TL;DR: The LAMP method presented here uses loop primers to achieve reaction times of less than half that of the original LAMP, which should facilitate genetic analysis, including genetic diagnosis in the clinical laboratory.
Journal ArticleDOI
Foot-and-Mouth Disease
Marvin J. Grubman,Barry Baxt +1 more
TL;DR: The reemergence of FMD in developed countries that had been disease free for many years is described and the effect that this has had on disease control strategies is described.
Journal ArticleDOI
Molecular epidemiology of foot-and-mouth disease virus.
Nick J. Knowles,A. R. Samuel +1 more
TL;DR: For the first time, it is able to unequivocally show the recent pandemic spread of a FMDV type O strain through the whole of Asia and into Africa and Europe.
Journal ArticleDOI
Use of a portable real-time reverse transcriptase-polymerase chain reaction assay for rapid detection of foot-and-mouth disease virus.
Johnny D. Callahan,Fred Brown,Fernando A. Osorio,Jung H Sur,Ed Kramer,Gary W Long,Juan Lubroth,Stefanie J Ellis,Katina S Shoulars,Kristin L Gaffney,Daniel L. Rock,William M. Nelson +11 more
TL;DR: The RT-PCR test can be performed in 2 hours or less on a portable instrument, thus providing a rapid, portable, sensitive, and specific method for detection of FMDV.