Journal ArticleDOI
Development of a microfluidic biosensor for detection of environmental mycobacteria
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TLDR
A microfluidic chip to selectively culture mycolic acid containing actinomycetes from environmental samples will improve screening for mycobacteria to protect public health as well as screening forMycob bacteria to reduce the costs associated with treating nocardiafoam at sewage treatment plants.Abstract:
In this paper, we describe the development of a microfluidic culture-based biosensor for detecting mycobacteria in environmental samples. The biochips rely upon the unique paraffinophilic (i.e., wax-loving) nature of mycobacteria to rapidly and selectively differentiate them from non-target microorganisms. A new method of depositing and patterning paraffin was developed to fabricate the prototype biosensors using Opticlear™ as solvent to generate liquid paraffin and pattering with positive photoresist lift-off. The prototype biochips were experimentally tested to demonstrate the concept of rapid and selective detection of mycobacteria using pure cultures and epifluorescence microscopy to visualize microorganisms on the surface. Our successful demonstration of the culture-based biochip technology presents an alternative approach for developing new technology to track microorganisms in complex environmental samples. A microfluidic chip to selectively culture mycolic acid containing actinomycetes from environmental samples will improve screening for mycobacteria to protect public health as well as screening for mycobacteria to reduce the costs associated with treating nocardiafoam at sewage treatment plants.read more
Citations
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Journal ArticleDOI
Microfluidics Integrated Biosensors: A Leading Technology towards Lab-on-a-Chip and Sensing Applications
George Luka,Ali Ahmadi,Homayoun Najjaran,Evangelyn C. Alocilja,Maria C. DeRosa,Kirsten R. Wolthers,Ahmed Malki,Hassan A. Aziz,Asmaa Althani,Asmaa Althani,Mina Hoorfar +10 more
TL;DR: The integration of microfluidic and biosensor technologies provides the ability to merge chemical and biological components into a single platform and offers new opportunities for future biosensing applications including portability, disposability, real-time detection, unprecedented accuracies, and simultaneous analysis of different analytes in a single device.
Journal ArticleDOI
Microfluidic-integrated biosensors: prospects for point-of-care diagnostics.
Suveen Kumar,Saurabh Kumar,Md. Azahar Ali,Md. Azahar Ali,Pinki Anand,Ved Varun Agrawal,Renu John,Sagar Maji,Bansi D. Malhotra,Bansi D. Malhotra +9 more
TL;DR: This review focuses on the latest advancements in the fields of microfluidic biosensing technologies, and on the challenges and possible solutions for translation of this technology for POC diagnostic applications.
Journal ArticleDOI
Microsystems for biofilm characterization and sensing - A review.
TL;DR: This review presents some of the current developments in microsystems for characterization and sensing of bacterial biofilms and shows how these devices can lead to better understanding of the physiology and function of these communities of bacteria, which can eventually lead to the development of novel treatments that do not rely on high-dosage antibiotics.
Journal ArticleDOI
Room temperature UV adhesive bonding of CE devices
TL;DR: A simple low temperature adhesive 'stamp-and-stick' bonding procedure for lab-on-a-chip glass devices has been tested for capillary electrophoresis applications and exhibited excellent chip-to-chip reproducibility.
Proceedings ArticleDOI
Design and simulation of a Micro Hotplate for MEMS based integrated gas sensing system
TL;DR: Design and simulation of uniform temperature MHP has been investigated which is best suitable for sensing multiple gases by using array of MHP to get the better sensitivity and better selectivity.
References
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Book
Standard methods for the examination of water and wastewater
TL;DR: The most widely read reference in the water industry, Water Industry Reference as discussed by the authors, is a comprehensive reference tool for water analysis methods that covers all aspects of USEPA-approved water analysis.
Book
CRC Handbook of Chemistry and Physics
TL;DR: CRC handbook of chemistry and physics, CRC Handbook of Chemistry and Physics, CRC handbook as discussed by the authors, CRC Handbook for Chemistry and Physiology, CRC Handbook for Physics,
Journal ArticleDOI
Polymer microfluidic devices
Holger Becker,Laurie E. Locascio +1 more
TL;DR: This article presents a review of polymer-based microfluidic systems including their material properties, fabrication methods, device applications, and finally an analysis of the market that drives their development.
Journal ArticleDOI
Mycobacterium avium complex infection in the acquired immunodeficiency syndrome.
TL;DR: Before the acquired immunodeficiency syndrome (AIDS) epidemic, disseminated infection with M. avium complex was extremely rare; by 1980, only 24 cases had been reported in the medical literature; beginning in 1982, however, the number of cases increased dramatically.
Journal ArticleDOI
Polymer microfabrication methods for microfluidic analytical applications
Holger Becker,Claudia Gärtner +1 more
TL;DR: This paper presents an overview of existing polymer microfabrication technologies for microfluidic applications, namely replication methods such as hot embossing, injection molding and casting, and the technologies necessary to fabricate the molding masters.