Journal ArticleDOI
Developmental and environmental concurrent expression of sunflower dry-seed-stored low-molecular-weight heat-shock protein and Lea mRNAs.
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TLDR
The coordinate accumulation of Lea and lmw-HSP transcripts during embryo development and in response to stress and ABA suggests the existence of common regulatory elements for Lea, and supports the notion that HSPs might have alternative functions in the plant cell.Abstract:
We have cloned and sequenced three different cDNAs from sunflower seed-stored mRNA. Sequence similarities and response to heat-shock identified one of the cDNAs as a low-molecular-weight heat-shock protein (lmw-HSP). The other two clones showed significant sequence similarity to the cotton and carrot late-embryogenesis-abundant (Lea) proteins D-113 and Emb-1, respectively. The three cDNAs showed similar expression patterns during zygotic embryo development, as well as in vegetative tissues of 3-day-old seedlings in response to stress. Maximal accumulation of all three mRNAs was detected in dry seeds and during embryo mid-maturation stage, in the absence of exogenous stress. In seedlings, mRNAs accumulated to lower levels in response to osmotic stress and exogenous abscisic acid (ABA) treatments. A differential time course of response to osmotic stress was observed: lmw-HSP mRNA accumulation was induced earlier than that of Lea mRNAs. The coordinate accumulation of Lea and lmw-HSP transcripts during embryo development and in response to stress and ABA suggests the existence of common regulatory elements for Lea and lmw-HSP genes, and supports the notion that HSPs might have alternative functions in the plant cell.read more
Citations
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Journal ArticleDOI
The molecular basis of dehydration tolerance in plants
J. Ingram,Dorothea Bartels +1 more
TL;DR: A large number of genes with a potential role in drought tolerance have been described, and major themes in the molecular response have been established.
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Mechanisms of plant desiccation tolerance.
TL;DR: Recent advances in the understanding of the mechanism of anhydrobiosis include the downregulation of metabolism, dehydration-induced partitioning of amphiphilic compounds into membranes and immobilization of the cytoplasm in a stable multicomponent glassy matrix.
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Physiology and molecular biology of salinity stress tolerance in plants
Raj Kumar Sairam,Aruna Tyagi +1 more
TL;DR: Salinity stress response is multigenic, as a number of processes affected, such as various compatible solutes/osmolytes, polyamines, reactive oxygen species and antioxidant defence mecha- nism, ion transport and compartmentalization of inj u- rious ions.
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Molecular networks regulating Arabidopsis seed maturation, after-ripening, dormancy and germination
TL;DR: Current knowledge of the molecular control of this trait in Arabidopsis thaliana is presented, focussing on important components functioning during the developmental phases of seed maturation, after-ripening and imbibition.
Journal ArticleDOI
Evolution, structure and function of the small heat shock proteins in plants
TL;DR: Although the smHSPs are apparently not essential for basal cell functions as are the high molecular weight HSPs such as HSP90, HSP70 and HSP60, their functions are likely to be critical for survival and recovery from heat stress as well as for specific developmental processes.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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A comprehensive set of sequence analysis programs for the VAX
TL;DR: A group of programs that will interact with each other has been developed for the Digital Equipment Corporation VAX computer using the VMS operating system.
Journal ArticleDOI
The heat-shock proteins
Susan Lindquist,E A Craig +1 more
TL;DR: Roles moleculaires des proteines de choc thermique dans le fonctionnement des organismes a des temperatures normales et suite a des chocs thermiques; differents genes impliques.
Journal ArticleDOI
The Roles of Heat Shock Proteins in Plants
TL;DR: HSP70; CHARACTERISTICS and FUNCTION S 582 HSP70 Diversity.
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Selection of AUG initiation codons differs in plants and animals.
TL;DR: Structural and functional findings suggest that the factors which select AUG initiation codons in plants and animals differ significantly, and that the consensus sequence for animals was CACCAUG that for plants was AACAAUGGC.