Journal ArticleDOI
Sequence and characterization of 6 Lea proteins and their genes from cotton.
TLDR
The amino acid composition and sequence for the Lea proteins are unusual, five are extremely hydrophilic, four contain no cys or trp and 4 have sequence domains that suggest amphiphilic helical structures.Abstract:
Lea genes code for mRNAs and proteins that are late embryogenesis abundant in higher plant seed embryos. They appear to be ubiquitous in higher plants and may be induced to high levels of expression in other tissues and at other times of ontogeny by ABA and/or desiccation. Presented here are the genomic and cDNA sequences for 6 of these genes from cotton seed embryos and the derived amino acid sequences of the corresponding proteins. The Lea genes contain the standard sequence features of eucaryotic genes (TATA box and poly (A) addition sequences) and have 1 or more introns. Sequences differences between cDNA and genomic DNA confirm the existence of small multigene families for several Lea genes. The amino acid composition and sequence for the Lea proteins are unusual. Five are extremely hydrophilic, four contain no cys or trp and 4 have sequence domains that suggest amphiphilic helical structures. Hypothetical functions in desiccation survival, based on amino acid sequence, are discussed.read more
Citations
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Journal ArticleDOI
The molecular basis of dehydration tolerance in plants
J. Ingram,Dorothea Bartels +1 more
TL;DR: A large number of genes with a potential role in drought tolerance have been described, and major themes in the molecular response have been established.
Journal ArticleDOI
Cold Acclimation and Freezing Stress Tolerance: Role of Protein Metabolism
TL;DR: The Kinetics of Cold Acclimation and Deacclimation, and the Development of Inducible Response, are studied.
Journal ArticleDOI
Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice
TL;DR: This study provides direct evidence supporting the hypothesis that LEA proteins play an important role in the protection of plants under water-or salt-stress conditions and finds that the extent of increased stress tolerance correlated with the level of the HVA1 protein accumulated in the transgenic rice plants.
Journal ArticleDOI
Gene expression in response to abscisic acid and osmotic stress.
Karen Skriver,John Mundy +1 more
TL;DR: Several ABA-responsive genes have been isolated and a major goal of the research discussed below is to understand the role these genes play in osmotic stress and desiccation tolerance.
Journal ArticleDOI
Common amino acid sequence domains among the LEA proteins of higher plants.
Leon Dure,Martha L. Crouch,John J. Harada,Tuan-Hua David Ho,John Mundy,Ralph S. Quatrano,Terry L. Thomas,Zinmay Renee Sung +7 more
TL;DR: Analysis of conserved tracts of amino acid sequence among LEA proteins from several species suggests that it exists as an amphiphilic α helix which may serve as the basis for higher order structure.
References
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A simple method for displaying the hydropathic character of a protein
Jack Kyte,Russell F. Doolittle +1 more
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TL;DR: This paper discusses the physical properties of polypeptides, the structure of which has been determined Crystallographically to High Resolution and its role in the biosynthesis of Proteins.
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Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids
Arnold J. Berk,Phillip A. Sharp +1 more
TL;DR: A simple and sensitive method for detecting, sizing and mapping RNA transcripts from viral or cloned DNAs has been developed and used to examine the cytoplasmic transcripts produced during the early phase of adenovirus 2 (Ad2) infection of HeLa cells.
Journal ArticleDOI
Patterns of Amino Acids near Signal‐Sequence Cleavage Sites
TL;DR: In this paper, some such patterns, based on a sample of 78 eukaryotic signal sequences, are presented and discussed, and a first attempt at formulating rules for the prediction of cleavage sites is made.