Difference between N-acetylgalactosamine 4-sulfate 6-O-sulfotransferases from human serum and squid cartilage in specificity toward the terminal and interior portion of chondroitin sulfate.
TLDR
It appears that the role of the squid sulfotransferase is to synthesize so-called chondroitin sulfate E where over 50% of the interior hexosamine units are 4,6-bis-sulfated.About:
This article is published in Journal of Biological Chemistry.The article was published on 1986-04-05 and is currently open access. It has received 20 citations till now. The article focuses on the topics: Chondroitin sulfate & Dermatan sulfate.read more
Citations
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Journal ArticleDOI
Chemical and Immunological Assay of the Nonreducing Terminal Residues of Chondroitin Sulfate from Human Aggrecan
Anna Plaas,Anna Plaas,Shirley Wong-Palms,Peter J. Roughley,Ronald J. Midura,Vincent C. Hascall +5 more
TL;DR: The quantitative results obtained from chemical analyses of all nonreducing termini of aggrecan chondroitin sulfate chains revealed important changes in chain termination that occur when cellular activities are altered as adult articular cartilage is formed after removal of growth cartilage.
Journal ArticleDOI
Human N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase cDNA Is Related to Human B Cell Recombination Activating Gene-associated Gene
TL;DR: Cl cloning and characterization of human GalNAc4S-6ST is reported, which contains a single open reading frame that predicts a type II transmembrane protein composed of 561 amino acid residues.
Journal ArticleDOI
Influence of chlorate on proteoglycan biosynthesis by cultured human fibroblasts.
TL;DR: The influence of chlorate, an inhibitor of sulfate adenylyltransferase, on biosynthesis and secretion of proteoglycans was investigated in cultured human skin fibroblasts and may be considered as a means for the production of proteglycans with defined structural alterations.
Journal ArticleDOI
Purification of chondroitin 6-sulfotransferase secreted from cultured chick embryo chondrocytes.
TL;DR: Chondroitin 6-sulfotransferase, which transfers sulfate from 3'-phosphoadenylyl sulfate to position 6 of N-acetylgalactosamine in chondroitIn, was purified 1,430-fold to apparent homogeneity with a 22% yield from the serum-free culture medium of chick embryo chondrocytes by affinity chromatography on heparin-Sepharose CL-6B, wheat germ agglut
Journal ArticleDOI
Chondroitin sulfate synthase-2. Molecular cloning and characterization of a novel human glycosyltransferase homologous to chondroitin sulfate glucuronyltransferase, which has dual enzymatic activities.
Toshikazu Yada,Masanori Gotoh,Masanori Gotoh,Takashi Sato,Masafumi Shionyu,Mitiko Go,Hiromi Kaseyama,Hiroko Iwasaki,Hiroko Iwasaki,Norihiro Kikuchi,Yeon-Dae Kwon,Akira Togayachi,Akira Togayachi,Takashi Kudo,Hideto Watanabe,Hisashi Narimatsu,Koji Kimata +16 more
TL;DR: Results indicate that this enzyme could synthesize chondroitin sulfate chains as a chondDetroit in sulfate synthase that has both glucuronyltransferase and N-acetylgalactosaminyltransferase activities.
References
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.
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Formation of Three Types of Disulfated Disaccharides from Chondroitin Sulfates by Chondroitinase Digestion
Sakaru Suzuki,Sakaru Suzuki,Hidehiko Saito,Hidehiko Saito,Tatsuya Yamagata,Tatsuya Yamagata,Kimiko Anno,Kimiko Anno,Nobuko Seno,Nobuko Seno,Yumiko Kawai,Yumiko Kawai,Tamotsu Furuhashi,Tamotsu Furuhashi +13 more
TL;DR: Three isomeric disulfated disaccharides were separated from nonsulfated and monosulfated homologues and from one another by paper chromatography in 1-butyric acid-0.5 n ammonia (5:3).
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Culture from mouse bone marrow of a subclass of mast cells possessing a distinct chondroitin sulfate proteoglycan with glycosaminoglycans rich in N-acetylgalactosamine-4,6-disulfate.
TL;DR: The 35S]labeled proteoglycan of these unique IgE receptor-bearing and histamine-containing cells is composed of chondroitin sulfate E rather than heparin glycosaminoglycan, and thus is the first identification of such an intracellular localized proteogly can in a mammalian cell.
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Crystallization and some properties of chondroitinase from Arthrobacter aurescens.
TL;DR: A strain of Arthrobacter aurescens which secretes a large amount of chondroitinase into a culture broth, was isolated from soil and it is suggested that the chond Detroitinase is a chondDetroitin sulfate A and C lyase, one of the hyaluronate lyases.
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Structure of dermatan sulfate. I. Degradation by testicular hyaluronidase.
Lars-Åke Fransson,Lennart Rodén +1 more
TL;DR: It is demonstrated that highly purified dermatan sulfate is partially degraded by testicular hyaluronidase yielding polysaccharide fragments with d-glucuronic acid in the newly formed nonreducing termini.