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Open AccessJournal ArticleDOI

Digital holography of total internal reflection

William M. Ash, +1 more
- 23 Jun 2008 - 
- Vol. 16, Iss: 13, pp 9811-9820
TLDR
This work introduces a new microscopy technique termed total internal reflection holographic microscopy (TIRHM), which uses quantitative phase microscopy by digital holography to image the phase profile of light in totalinternal reflection, which is modulated by the materials present on or near the surface of internal reflection.
Abstract
We introduce a new microscopy technique termed total internal reflection holographic microscopy (TIRHM). Quantitative phase microscopy by digital holography is used to image the phase profile of light in total internal reflection, which is modulated by the materials present on or near the surface of internal reflection. The imaging characteristics are theoretically modeled and imaging capabilities are experimentally demonstrated.

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Citations
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Journal ArticleDOI

Principles and techniques of digital holographic microscopy

TL;DR: Digital holography is an emerging field of new paradigm in general imaging applications as discussed by the authors, and a review of a subset of the research and development activities in digital holographic microscopy techniques and applications is presented.
Journal ArticleDOI

Quantitative phase imaging techniques for the study of cell pathophysiology: from principles to applications.

TL;DR: The principles of QPI are presented and some of the recent applications ranging from cell homeostasis to infectious diseases and cancer are highlighted, to provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology.
Journal ArticleDOI

Digital holography and its multidimensional imaging applications: a review.

TL;DR: Digital holography can be used to perform multidimensional imaging of three-dimensional structure, dynamics, quantitative phase, multiple wavelengths, and polarization state of light and sensing of a holographic image of nonlinear light and a three- dimensional image of incoherent light.

총회초청강연 I : Digital Holographic Microscopy

TL;DR: A survey of digital holographic and interferometric principles for quantitative phase microscopy can be found in this paper, where a brief background on optical microscopy is given. And a survey is given of the wide and very active field of research in digital holography techniques and applications.
Book ChapterDOI

Digital Holographic Microscopy

TL;DR: A survey is given of the wide and very active field of research in DHM techniques and applications and the basis of many other techniques of QPM with novel capabilities are given.
References
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Journal ArticleDOI

Phase-shifting digital holography

TL;DR: A new method is proposed in which the distribution of complex amplitude at a plane is measured by phase-shifting interferometry and then Fresnel transformed by a digital computer, which can reconstruct an arbitrary cross section of a three-dimensional object with higher image quality and a wider viewing angle than from conventional digital holography using an off-axis configuration.
Journal ArticleDOI

Direct recording of holograms by a CCD target and numerical reconstruction.

TL;DR: The principle of recording holograms directly on a CCD target is described and a real image of the object is reconstructed from the digitally sampled hologram by means of numerical methods.
Journal ArticleDOI

Digital holography for quantitative phase-contrast imaging.

TL;DR: A new application of digital holography for phase-contrast imaging and optical metrology and an application to surface profilometry shows excellent agreement with contact-stylus probe measurements.
Journal ArticleDOI

Cell-substrate contacts illuminated by total internal reflection fluorescence.

TL;DR: Total internal reflection fluorescence examination of cells appears to have promising applications, including visualization of the membrane and underlying cytoplasmic structures at cell-substrate contacts, dramatic reduction of autofluorescence from debris and thick cells, mapping of membranes topography, and visualization of reversible bound fluorescent ligands at membrane receptors.
Journal ArticleDOI

High-resolution quantitative phase-contrast microscopy by digital holography

TL;DR: Techniques of digital holography are improved in order to obtain high-resolution, high-fidelity images of quantitative phase-contrast microscopy, and the angular spectrum method of calculating holographic optical field is seen to have significant advantages including tight control of spurious noise components.
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