Double-Stranded Regions in Heterogeneous Nuclear RNA from Hela Cells
TLDR
Heterogeneous nuclear RNA from HeLa cells contains double-stranded regions that arise by base pairing of complementary sequences that exist as parts of the same molecule (intramolecular base pairing).Abstract:
Heterogeneous nuclear RNA from HeLa cells contains double-stranded regions that arise by base pairing of complementary sequences that exist as parts of the same molecule (intramolecular base pairing). When denatured, the RNA sequences that form the double-stranded regions hybridize rapidly to HeLa cell DNA, suggesting that they are transcribed from reiterated sites in the genome. The messenger RNA does not contain the same class or amount of double-stranded RNA regions found in heterogeneous nuclear RNA.read more
Citations
More filters
Journal ArticleDOI
The Alu family of dispersed repetitive sequences
Carl W. Schmid,Warren R. Jelinek +1 more
TL;DR: Property of this repeat sequence, its flanking sequences in chromosomal DNA, and RNA's transcribed from it suggest that it may be a mobile DNA element inserted at hundreds of thousands of different chromosomal locations.
Journal ArticleDOI
Heterogeneous nuclear RNA-protein fibers in chromatin-depleted nuclei.
R Herman,L Weymouth,S Penman +2 more
TL;DR: It is suggested that hnRNA metabolism does not take place in a soluble nucleoplasmic compartment but on organized structures firmly bound to the nuclear structure.
Journal ArticleDOI
Alternative splicing caused by RNA secondary structure
TL;DR: Both in extracts and in cells, an exon became optional when sequestered in a hairpin loop, and the same types of alternatively spliced RNAs were formed when a similar template was introduced into HeLa cells by transfection.
Journal ArticleDOI
Flow cytofluorometric analysis of cell cycle distributions using propidium iodide. Properties of the method and mathematical analysis of the data.
TL;DR: The new rapid staining method proposed by Krishan is useful for the measurement of relative DNA content by flow cytofluorometry, although modifications in the technique are necessary for some cell types which grow in monolayers.