Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth.
Kim Boutilier,Kim Boutilier,Remko Offringa,Vijay K. Sharma,Henk Kieft,Thérèse Ouellet,Lemin Zhang,Jiro Hattori,Chun-Ming Liu,André A. M. van Lammeren,Brian Miki,Jan Custers,Michiel M. Van Lookeren Campagne +12 more
TLDR
The expression pattern of BABY BOOM in developing seeds combined with the BBM overexpression phenotype suggests a role for this gene in promoting cell proliferation and morphogenesis during embryogenesis.Abstract:
The molecular mechanisms underlying the initiation and maintenance of the embryonic pathway in plants are largely unknown. To obtain more insight into these processes, we used subtractive hybridization to identify genes that are upregulated during the in vitro induction of embryo development from immature pollen grains of Brassica napus (microspore embryogenesis). One of the genes identified, BABY BOOM (BBM), shows similarity to the AP2/ERF family of transcription factors and is expressed preferentially in developing embryos and seeds. Ectopic expression of BBM in Arabidopsis and Brassica led to the spontaneous formation of somatic embryos and cotyledon-like structures on seedlings. Ectopic BBM expression induced additional pleiotropic phenotypes, including neoplastic growth, hormone-free regeneration of explants, and alterations in leaf and flower morphology. The expression pattern of BBM in developing seeds combined with the BBM overexpression phenotype suggests a role for this gene in promoting cell proliferation and morphogenesis during embryogenesis.read more
Citations
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Genome-Wide Analysis of the ERF Gene Family in Arabidopsis and Rice
TL;DR: It was concluded that the major functional diversification within the ERF family predated the monocot/dicot divergence and might have been due to chromosomal/segmental duplication and tandem duplication, as well as more ancient transposition and homing.
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APETALA2/Ethylene Responsive Factor (AP2/ERF) transcription factors: mediators of stress responses and developmental programs
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WRINKLED1 encodes an AP2/EREB domain protein involved in the control of storage compound biosynthesis in Arabidopsis
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TL;DR: Oil-accumulating seedlings showed aberrant development consistent with a prolonged embryonic state, and the putative AP2/EREBP transcription factor WRINKLED1 (WRI1) is involved in the regulation of seed storage metabolism in Arabidopsis.
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Plant Callus: Mechanisms of Induction and Repression
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Selectable marker genes in transgenic plants: applications, alternatives and biosafety
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TL;DR: This review of marker genes used for transgenic and transplastomic plant research or crop development has been assessed for efficiency, biosafety, scientific applications and commercialization and two have emerged with significant potential.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
Steven J. Clough,Andrew F. Bent +1 more
TL;DR: The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T-DNA gene tagging, positional cloning, or attempts at targeted gene replacement.
Journal ArticleDOI
Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis.
Qiang Liu,Mie Kasuga,Yoh Sakuma,Hiroshi Abe,Setsuko Miura,Kazuko Yamaguchi-Shinozaki,Kazuo Shinozaki +6 more
TL;DR: Overexpression of the DREB1A cDNA in transgenic Arabidopsis plants not only induced strong expression of the target genes under unstressed conditions but also caused dwarfed phenotypes in the transgenic plants, and revealed freezing and dehydration tolerance.
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