Journal ArticleDOI
Effect of cytosine arabinoside 5'-triphosphate on mammalian DNA polymerase.
TLDR
About 90% of the radio-activity in the DNA product after treatment with micrococcal nuclease and spleen phosphodiesterase was released as 3H-cytosine arabinoside, suggesting that most of the incorporation was at the 3′-hydroxyl terminal end and not within the polydeoxynucleotide chain.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1969-02-21. It has received 134 citations till now. The article focuses on the topics: DNA polymerase & DNA polymerase II.read more
Citations
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Journal ArticleDOI
Correlation of cytotoxicity with incorporation of ara-C into DNA.
TL;DR: The results suggest that the incorporation of ara-C into DNA is one mechanism responsible for producing lethal cellular events.
Journal ArticleDOI
Effects of hydroxyurea and 1-beta-D-arabinofuranosyl-cytosine on deoxyribonucleotide pools in mouse embryo cells.
Lambert Skoog,Bo Nordenskjöld +1 more
TL;DR: The results show that the inhibition of DNA synthesis by hydroxyurea is correlated with diminishing pools of dGTP and dATP and a decreased turnover of dTTP, indicating a generally decreased deoxyribonucleotide formation presumably due to the inhibited ribonucleoside-diphosphate reductase.
Book ChapterDOI
Ara-C: Cellular and Molecular Pharmacology
TL;DR: The antimetabolite cytosine arabinoside (ara-C) represents a prototype of the nucleoside analog class of antineoplastic agents and remains one of the most effective drugs used in the treatment of acute leukemia as well as other hematopoietic malignancies.
Journal Article
The effect of-beta-D-arabinofuranosylcytosine on growth, viability, and DNA synthesis of mouse L-cells.
F. L. Graham,G. F. Whitmore +1 more
TL;DR: Results of studies on growth and cell viability suggest that at higher concentrations ara-C rapidly killed S phase cells but temporarily blocked the progression of cells from G 1 into S, and thus partially protected against its own toxicity, and agrees with a model in which inhibition of DNA synthesis is the result of inhibition ofDNA polymerase.
Journal ArticleDOI
The clinical pharmacology of antineoplastic agents (second of two parts).
TL;DR: (Second of Two Parts) Cytidine and Deoxycytidine Analogues The pyrimidine nucleoside analogues cytosine arabinoside (cytarabine) and 5-azacytidine are of importance in the treatment of acute granul...
References
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Journal ArticleDOI
Enzymatic Synthesis of Deoxyribonucleic Acid. VIII. Frequencies of Nearest Neighbor Base Sequences in Deoxyribonucleic Acid
TL;DR: In the studies to be reported here, the frequencies of the 16 possible nearest neighbor pairs in a variety of DNA’s are derived by the technique of enzymatic incorporation of 5'-Pazlabeled nucleotides into DNA and then degradation of the DNA into 3’-nucleotides.
Journal Article
Inhibition of mammalian DNA polymerase by the 5'-triphosphate of 1-beta-d-arabinofuranosylcytosine and the 5'-triphosphate of 9-beta-d-arabinofuranoxyladenine.
J. J. Furth,Seymour S. Cohen +1 more
TL;DR: Studies indicate that the inhibition of mammalian DNA polymerase by the 5′-triphosphate of 9-β-d-arabinofuranosylcytosine (ara-ATP) is also competitive with the corresponding deoxytriphosphates.
Journal ArticleDOI
The incorporation of 3H-cytosine arabinoside and its effect on murine leukemic cells (L5178Y).
Chu Ming-Yu,Glenn A. Fischer +1 more
TL;DR: Murine leukemic cells, L5178Y, inhibited by a low level of cytosine arabino-side for 6 hr could be rescued by deoxycytidine, and the incorporation into RNA could be correlated with irreversible inhibition of cell reproduction.
Related Papers (5)
Inhibition of mammalian DNA polymerase by the 5'-triphosphate of 1-beta-d-arabinofuranosylcytosine and the 5'-triphosphate of 9-beta-d-arabinofuranoxyladenine.
J. J. Furth,Seymour S. Cohen +1 more
Studies in mouse L-cells on the incorporation of 1-beta-D-arabinofuranosylcytosine into DNA and on inhibition of DNA polymerase by 1-beta-D-arabinofuranosylcytosine 5'-triphosphate.
Frank L. Graham,G.F. Whitmore +1 more
The effect of-beta-D-arabinofuranosylcytosine on growth, viability, and DNA synthesis of mouse L-cells.
F. L. Graham,G. F. Whitmore +1 more