Open AccessJournal Article
The effect of-beta-D-arabinofuranosylcytosine on growth, viability, and DNA synthesis of mouse L-cells.
F. L. Graham,G. F. Whitmore +1 more
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Results of studies on growth and cell viability suggest that at higher concentrations ara-C rapidly killed S phase cells but temporarily blocked the progression of cells from G 1 into S, and thus partially protected against its own toxicity, and agrees with a model in which inhibition of DNA synthesis is the result of inhibition ofDNA polymerase.Abstract:
Summary An examination of the effects of 1-β-d-arabinosylcytosine (ara-C) on DNA synthesis of mouse L-cells has been made, and these effects have been compared with effects on viability. Measurements on the incorporation of radioactive precursors into acid-insoluble material showed that ara-C strongly inhibited DNA synthesis while having relatively little effect on RNA or protein synthesis. It was found that 3.6 × 10 -7 m ara-C inhibited cell division for at least 24 hr, except for the single division of cells initially in G 2 . Over this time, however, there was no decrease in cell viability, even though DNA synthesis was reduced by more than 97% over the first 14 hr of exposure to ara-C. Results of studies on growth and cell viability suggest that at higher concentrations (7.2 × 10 -6 m or greater) ara-C rapidly killed S phase cells but temporarily blocked the progression of cells from G 1 into S, and thus partially protected against its own toxicity. The observed effects are discussed in relation to the current models for the mechanism of action of ara-C. Our observations do not appear to be consistent with a model in which ara-C acts by blocking the reduction of CDP nor with one in which ara-C acts by incorporation into DNA. Rather, our results agree with a model in which inhibition of DNA synthesis is the result of inhibition of DNA polymerase.read more
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References
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Journal ArticleDOI
Plaque formation and isolation of pure lines with poliomyelitis viruses.
Renato Dulbecco,Marguerite Vogt +1 more
TL;DR: Pure virus lines were established by isolating the virus population produced in single plaques, which had the same morphological, serological, and pathogenic properties as the parent strain.
Journal ArticleDOI
Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.
TL;DR: Application of the methods described in this paper as a tool for quantitative study of normal mammalian cell growth, physiology, genetics, and biochemistry, and the response of cells to drugs, viruses, high energy radiation, and other agents have been indicated.
Journal Article
Inhibition of mammalian DNA polymerase by the 5'-triphosphate of 1-beta-d-arabinofuranosylcytosine and the 5'-triphosphate of 9-beta-d-arabinofuranoxyladenine.
J. J. Furth,Seymour S. Cohen +1 more
TL;DR: Studies indicate that the inhibition of mammalian DNA polymerase by the 5′-triphosphate of 9-β-d-arabinofuranosylcytosine (ara-ATP) is also competitive with the corresponding deoxytriphosphates.
Journal ArticleDOI
The incorporation of 3H-cytosine arabinoside and its effect on murine leukemic cells (L5178Y).
Chu Ming-Yu,Glenn A. Fischer +1 more
TL;DR: Murine leukemic cells, L5178Y, inhibited by a low level of cytosine arabino-side for 6 hr could be rescued by deoxycytidine, and the incorporation into RNA could be correlated with irreversible inhibition of cell reproduction.
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Studies in mouse L-cells on the incorporation of 1-beta-D-arabinofuranosylcytosine into DNA and on inhibition of DNA polymerase by 1-beta-D-arabinofuranosylcytosine 5'-triphosphate.
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Inhibition of mammalian DNA polymerase by the 5'-triphosphate of 1-beta-d-arabinofuranosylcytosine and the 5'-triphosphate of 9-beta-d-arabinofuranoxyladenine.
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