Journal ArticleDOI
Effects of glutaraldehyde fixation on the structure of tight junctions. A quantitative freeze-fracture analysis
TLDR
The results indicate that the tight junction fibrils may be polymerization products caused by the formidable crosslinking effect of GA, and it is suggested that tight junctions in vivo are comprised of discrete integral proteins with stronger binding to the external side of the membrane or to opposing junctional proteins in the adjacent membrane.About:
This article is published in Journal of Ultrastructure Research.The article was published on 1979-08-01. It has received 104 citations till now. The article focuses on the topics: Tight junction.read more
Citations
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Journal ArticleDOI
A single gene product, claudin-1 or -2, reconstitutes tight junction strands and recruits occludin in fibroblasts.
TL;DR: Findings suggested that claudin-1 and -2 are mainly responsible for TJ strand formation, and that occludin is an accessory protein in some function of TJ strands.
Journal ArticleDOI
Molecular architecture of tight junctions.
Laura L. Mitic,James M. Anderson +1 more
TL;DR: Control of perijunctional actin may be the unifying mechanism for regulating paracellular permeability.
Journal ArticleDOI
Modulation of tight junction structure in blood-brain barrier endothelial cells. Effects of tissue culture, second messengers and cocultured astrocytes
Hartwig Wolburg,Jochen Neuhaus,Uwe Kniesel,B. Krauss,Eva-Maria Schmid,M. Ocalan,C. Farrell,W. Risau +7 more
TL;DR: In this article, the structure and function of tight junctions in primary cultures of bovine brain endothelial cells using quantitative freeze-fracture electron microscopy, and ion and inulin permeability.
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Tight junction regulation in the mammary gland.
TL;DR: The tight junction state appears to be closely linked to milk secretion, and anincrease in tight junction permeability is accompanied by decrease in the milk secretion rate, and conversely, a decrease in tight junctions permeability are accompanied by an increase in the Milk secretion rate.
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Evidence for the lipidic nature of tight junction strands
Bechara Kachar,Thomas S. Reese +1 more
TL;DR: The proposition that membrane lipids1–4, rather than intrinsic membrane proteins5–7, are the principal structural elements of the strands comprising tight junctions is examined, which suggests that tight junction formation requires a phase transition of the planar lipid bilayer similar to that invoked in models of membrane fusion.
References
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Fracture faces of zonulae occludentes from "tight" and "leaky" epithelia
TL;DR: Epithelia of intermediate permeabilities exhibited junctions with intermediate or variable morphology, finding that the zonula occludens from a "very leaky" epithelium, the proximal convoluted tubule of the mouse kidney, is extremely shallow in the apical-basal direction, and consists of five or more interconnected junctional strands interposed between luminal and lateral membrane surfaces.
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Morphological factors influencing transepithelial permeability: a model for the resistance of the zonula occludens.
TL;DR: Evidence is reviewed here that indicates that in a number of simple epithelia the structure of thezonula occludens is largely responsible for the magnitude of transepithelial conductance.
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Further Observations on the Fine Structure of Freeze-Cleaved Tight Junctions
TL;DR: Evidence is presented suggesting that fragments of tight junctions can be internalized and broken down in lysosome-like vesicles.
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Segmental differentiations of cell junctions in the vascular endothelium. The microvasculature.
TL;DR: Small vascular units consisting of an arteriole, its capillaries, and the emerging venule (ACV units) were identified in the rat omentum and mesentery and it was shown that the intercellular junctions of the vascular endothelium vary characteristically from one segment to another in the microvasculature.
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Segmental differentiations of cell junctions in the vascular endothelium. Arteries and veins.
TL;DR: A systematic survey of endothelial junctions in elastic and muscular arteries and in medium and large veins has been carried out in the rat using freeze-cleaved preparations, finding two unusual types of junctions reminiscent of the septate junctions found in the epithelia of invertebrates.