Efficient Regeneration of Vanda Coerulea, an Endangered Orchid Using Thidiazuron

TLDR: Efficient shoot regeneration of Vanda coerulea was achieved using thin shoot tip sections and thidiazuron and the highest percentage of protocorm-like bodies (95%) survived and ultimately produced healthy shoots with 2 – 3 leaves when subjected to a 4 week thid Diazuron treatment.

Abstract: Efficient shoot regeneration of Vanda coerulea was achieved using thin shoot tip sections and thidiazuron Protocorm-like bodies or proliferating shoot buds was observed when thin shoot tip sections were cultured on Vacin and Went's (VW) (1949) basal medium supplemented with 1135 µM thidiazuron The highest percentage of protocorm-like bodies (95%) survived and ultimately produced healthy shoots with 2 – 3 leaves when subjected to a 4 week thidiazuron treatment A culture period longer than 8 weeks with thidiazuron resulted in the formation of fasciated or distorted shoots Shoots produced roots when cultured on half strength VW basal medium supplemented with 1142 µM IAA The well rooted shoots were transferred to pots containing charcoal chips, coconut husk and broken tiles (2:2:1) and a 98% survival rate was achieved