Journal ArticleDOI
Energy metabolism of Saccharomyces cerevisiae discrepancy between ATP balance and known metabolic functions
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TLDR
The results indicate that the ATP required for maintaining the yeast alive, what would be considered maintenance energy "sensu stricto", is only a minor proportion of the ATP spent in non net-biosynthetic functions during growth.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1976-09-13. It has received 96 citations till now. The article focuses on the topics: Fermentation & Catabolism.read more
Citations
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In vivo measurement of cytosolic and mitochondrial pH using a pH-sensitive GFP derivative in Saccharomyces cerevisiae reveals a relation between intracellular pH and growth.
TL;DR: In this article, the authors used the pH-sensitive GFP derivative ‘ratiometric pHluorin’ expressed in the cytosol and in the mitochondrial matrix of growing Saccharomyces cerevisiae to assess the variation in cytosolic pH (pHcyt) and mitochondrial pH(pHmit) in response to nutrient availability, respiratory chain activity, shifts in environmental pH and stress induced by addition of sorbic acid.
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Energetics of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat cultures.
TL;DR: The energetics of Saccharomyces cerevisiae were studied in anaerobic glucose-limited chemostat cultures via an analysis of biomass and metabolite production and a linear correlation was found between the energy required to compensate for import of protons and the amount of acid added.
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A theoretical evaluation of growth yields of yeasts
TL;DR: Calculations for growth of both yeasts on glucose, ethanol, and acetate made clear that only by assuming a fixed difference between theoretical and experimental ATP requirements, the P/O-ratios are more or less independent of the growth substrate.
Journal ArticleDOI
A metabolic network stoichiometry analysis of microbial growth and product formation
W.M. van Gulik,J. J. Heijnen +1 more
TL;DR: It is concluded that analysis of metabolic network stoichiometry is a useful tool to detect metabolic limits and to guide process intensification studies.
References
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Journal ArticleDOI
A theoretical study on the amount of ATP required for synthesis of microbial cell material
TL;DR: In previous studies on the relation between ATP production and formation of cell material these effects have unjustly been neglected, and it is concluded that in the literature evidence is presented that these parameters may vary under different growth conditions.
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Utilization of energy for growth and maintenance in continuous and batch cultures of microorganisms: A reevaluation of the method for the determination of ATP production by measuring molar growth yields
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Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources.
ES Polakis,W. Bartley +1 more
TL;DR: Glucose was much more effective in inducing the anaerobic pathways than was galactose and the 2-oxoglutarate-oxidase system was found to be the least active enzyme of the citric acid cycle.
Journal ArticleDOI
Energetics of the budding cycle of Saccharomyces cerevisiae during glucose limited aerobic growth.
TL;DR: The gas exchange of populations of Saccharomyces cerevisiae synchronized at different growth rates was determined and the specific oxygen uptake and carbon dioxide formation rate are shown to depend on the state of the cells in the budding cycle.
Journal ArticleDOI
Units of transcription and translation: the relationship between heterogeneous nuclear RNA and messenger RNA.
TL;DR: Lewin et al. as discussed by the authors discussed the evidence that relates hnRNA and mRNA, from kinetic data on mRNA production and analysis of the addition of polyadenylic acid to nu-clear RNA, and consider the limitations that can beplaced on the construction of models for a precur-sor-product relationship between hn RNA andmRNA.
Related Papers (5)
Utilization of energy for growth and maintenance in continuous and batch cultures of microorganisms: A reevaluation of the method for the determination of ATP production by measuring molar growth yields
Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources.
ES Polakis,W. Bartley +1 more