Journal ArticleDOI
Engineered Redox‐Responsive PEG Detachment Mechanism in PEGylated Nano‐Graphene Oxide for Intracellular Drug Delivery
Huiyun Wen,Chunyan Dong,Haiqing Dong,Aijun Shen,Wen-Juan Xia,Xiaojun Cai,Yanyan Song,Xue-Quan Li,Yongyong Li,Donglu Shi,Donglu Shi +10 more
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TLDR
A PEGylated nano-graphene oxide (NGO-SS-mPEG) with redox-responsive detachable PEG shell is developed that can rapidly release an encapsulated payload at tumor-relevant glutathione levels and inhibition of cell proliferation is directly correlated with increased intracellular GSH concentrations due to rapid DXR release.Abstract:
In biomedical applications, polyethylene glycol (PEG) functionalization has been a major approach to modify nanocarriers such as nano-graphene oxide for particular biological requirements. However, incorporation of a PEG shell poses a significant diffusion barrier that adversely affects the release of the loaded drugs. This study addresses this critical issue by employing a redox-responsive PEG detachment mechanism. A PEGylated nano-graphene oxide (NGO-SS-mPEG) with redox-responsive detachable PEG shell is developed that can rapidly release an encapsulated payload at tumor-relevant glutathione (GSH) levels. The PEG shell grafted onto NGO sheets gives the nanocomposite high physiological solubility and stability in circulation. It can selectively detach from NGO upon intracellular GSH stimulation. The surface-engineered structures are shown to accelerate the release of doxorubicin hydrochloride (DXR) from NGO-SS-mPEG 1.55 times faster than in the absence of GSH. Confocal microscopy shows clear evidence of NGO-SS-mPEG endocytosis in HeLa cells, mainly accumulated in cytoplasm. Furthermore, upon internalization of DXR-loaded NGO with a disulfide-linked PEG shell into HeLa cells, DXR is effectively released in the presence of an elevated GSH reducing environment, as observed in confocal microscopy and flow cytometric experiments. Importantly, inhibition of cell proliferation is directly correlated with increased intracellular GSH concentrations due to rapid DXR release.read more
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Nano-graphene in biomedicine: theranostic applications
TL;DR: Graphene-based photothermal therapy has been realized, achieving excellent anti-tumor therapeutic efficacy in animal experiments and future prospects and challenges of using graphene-based materials for theranostic applications are discussed.
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Carbon Nanomaterials for Biological Imaging and Nanomedicinal Therapy
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Graphene-based nanomaterials for drug delivery and tissue engineering.
TL;DR: This article presents a comprehensive review of various types and properties of graphene family nanomaterials and highlights how these properties are being exploited for drug delivery and tissue engineering applications.
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Graphene and graphene oxide as new nanocarriers for drug delivery applications.
TL;DR: An overview of recent advances in graphene-based nanocarriers, their biocompatibility and toxicity, followed by a summary of the most appealing examples demonstrated for the delivery of anti-cancer drugs and genes are presented.
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Toxicity of graphene-family nanoparticles: a general review of the origins and mechanisms
TL;DR: This review collects studies on the toxic effects of GFNs in several organs and cell models, and proposes some challenges and suggestions for further investigations of GFN toxicity, with the aim of completing the toxicology mechanisms and providing suggestions to improve the biological safety ofGFNs and facilitate their wide application.
References
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Journal ArticleDOI
PEGylated Nanographene Oxide for Delivery of Water-Insoluble Cancer Drugs
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