Journal ArticleDOI
Enhancement of d-glucuronolactone and acetaldehyde dehydrogenase activities in the rat liver by inducers of drug metabolism
Marios Marselos,Osmo Hänninen +1 more
TLDR
Three different fractions of d -glucuronolactone dehydrogenase were isolated from rat liver supernatant by means of CM and DEAE Sephadex ion exchange chromatography, and enhanced activity was obtained with both substrates, but it varied in degree within the group of animals.About:
This article is published in Biochemical Pharmacology.The article was published on 1974-05-15. It has received 28 citations till now. The article focuses on the topics: Glucuronolactone & Enzyme assay.read more
Citations
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Journal ArticleDOI
Different forms of rat liver aldehyde dehydrogenase and their subcellular distribution.
Timo Koivula,Martti Koivusalo +1 more
TL;DR: The properties and distribution of the NAD-linked unspecific aldehyde dehydrogenase activity has been studied in isolated cytoplasmic, mitochondrial and microsomal fractions of rat liver.
Journal ArticleDOI
Induction of different rat liver supernatant aldehyde dehydrogenases by phenobarbital and tetrachlorodibenzo-p-dioxin.
Journal ArticleDOI
Aryl hydrocarbon hydroxylase induction and its relationship to the toxicity of halogenated aryl hydrocarbons
A. Parkinson,Stephen Safe +1 more
Journal ArticleDOI
Induction of aldehyde dehydrogenases.
TL;DR: Rat liver contains two inducible aldehyde dehydrogenases in the cytosol, and phenobarbital, 1,1,1-trichloro-2,2-bis-(p-chlorophenylethane) (DDT), and dodecachlorooctahydro-1,3,4-metheno- 2H-cyclobuto[cd]pentalene (Mirex) induce only the φ enzyme significantly
Journal ArticleDOI
Effect of phenobarbital and 3-methylcholanthrene on aldehyde dehydrogenase activity in cultures of HepG2 cells and normal human hepatocytes.
TL;DR: It is concluded, that HepG2 hepatoma cells behave similar to the normal hepatocytes in terms of AL DH regulation and can be used for studies on the activity of ALDH as modified by added xenobiotics.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal Article
Adaptive increases in drug-metabolizing enzymes induced by phenobarbital and other drugs
TL;DR: Evidence is presented that phenobarbital induces the synthesis of these enzymes and stimulates liver protein synthesis, and administration of certain drugs enhances the ability of liver microsomes to metabolize the same or a closely related cornpound.
Journal Article
The metabolism of methylated aminoazo dyes. V. Evidence for induction of enzyme synthesis in the rat by 3-methylcholanthrene.
TL;DR: The ability of fortified rat liver homogenates to N-demethylate 3-methyl-4-monomethylaminoazobenzene (demethylase activity) and to reduce the azo linkage of 4-demethylaminozanthrene (reductaseActivity) was increased several-fold by the intraperitoneal injection of 0.1 to 10 mg.
Journal ArticleDOI
Horse Liver Aldehyde Dehydrogenase I. PURIFICATION AND CHARACTERIZATION
Rhoda I. Feldman,Henry Weiner +1 more
TL;DR: Horse liver aldehyde: NAD oxidoreductase (EC 1.2.1.3) has been purified to homogeneity by a procedure consisting of salt fractionation, ion exchange chromatography, and isoelectric focusing, suggesting a tetrameric structure for the native enzyme.