Journal ArticleDOI
Enzyme yields from cells of brewer's yeast disrupted by treatment in a horizontal disintegrator
Ference Marffy,Maria-Regina Kula +1 more
Reads0
Chats0
TLDR
Maltase, phosphatase, malate dehydrogenase, glucose‐6‐phosphate dehydrogensase, and 6-phosphogluconate dehydration activities were determined in the supernatant of brewer's yeast cells from samples taken at various intervals during a 90 min treatment in a Dyno‐Mill disintegrator.Abstract:
Maltase, phosphatase, malate dehydrogenase, glucose‐6‐phosphate dehydrogenase, and 6‐phosphogluconate dehydrogenase activities were determined in the supernatant of brewer's yeast cells from samples taken at various intervals during a 90 min treatment in a Dyno‐Mill disintegrator.read more
Citations
More filters
Journal ArticleDOI
Disruption of microbial cells for intracellular products
Yusuf Chisti,Murray Moo-Young +1 more
TL;DR: A review examines the state of the art of the large-scale cell disruption technology and disruption methods of potential commercial value in industry and medicine.
Journal ArticleDOI
Process-scale disruption of microorganisms.
TL;DR: In all cases it is essential to consider the interaction of the disruption operation with downstream units and to clearly demonstrate the cost benefits of alternative strategies.
Journal ArticleDOI
Purification and properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii.
TL;DR: Evidence is presented which demonstrates that the reaction product of the formaldehyde-dehydrogenase-catalyzed oxidation of formaldehyde is S-formylglutathione rather than formate.
Journal ArticleDOI
Mild disintegration of the green microalgae Chlorella vulgaris using bead milling
P.R. Postma,T.L. Miron,Giuseppe Olivieri,Maria J. Barbosa,René H. Wijffels,Michel H.M. Eppink +5 more
TL;DR: In this work, the mild disintegration of the microalgae Chlorella vulgaris for the release of intracellular products has been studied and selective extraction of water soluble proteins was observed as proteins released sooner than cell disintegration took place.
Journal ArticleDOI
Purification of Proteins and the Disruption of Microbial Cells
Maria-Regina Kula,Horst Schütte +1 more
TL;DR: Intracellular proteins with catalytic or biological activity are of growing importance for developments in enzyme technology, as well as for the production of mammalian proteins by recombinant‐DNA technology.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Release of enzymes from bakers' yeast by disruption in an industrial homogenizer
TL;DR: The rates of release of 7 enzymes from bakers' yeast have been measured and the disruption process did not cause loss of activity of these enzymes.
Journal ArticleDOI
Release of protein from Bakers' yeast (Saccharomyces cerevisiae) by disruption in an industrial agitator mill
TL;DR: Increased disruption efficiency was obtained at higher agitator speeds, greater loading of bead attritive elements and lower rates of upward recycle of yeast suspension through the mill.
Journal ArticleDOI
Disintegration of Microorganisms and Preparation of Yeast Cell Walls in a New Type of Disintegrator
J. Ŕeháćek,K. Beran,V. Bićík +2 more
TL;DR: Microbial cells were disintegrated in a new type of rotary disintegrator with a disc stirrer by a combination of shear force layers, collisions, and rolling of glass beads which were brought into motion by the stirrer.