Ethylene-Induced Stabilization of ETHYLENE INSENSITIVE3 and EIN3-LIKE1 Is Mediated by Proteasomal Degradation of EIN3 Binding F-Box 1 and 2 That Requires EIN2 in Arabidopsis
Fengying An,Qiong Zhao,Yusi Ji,Wenyang Li,Zhiqiang Jiang,Xiangchun Yu,Chen Zhang,Ying Han,Wenrong He,Yidong Liu,Shuqun Zhang,Joseph R. Ecker,Hongwei Guo +12 more
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TLDR
It is demonstrated that ethylene similarly regulates EIN3 and EIL1, the two master transcription factors coordinating myriad ethylene responses, and it is clarified that EIN2 but not MKK9 is required for ethylene-induced Ein3/EIL1 stabilization.Abstract:
Plant responses to ethylene are mediated by regulation of EBF1/2-dependent degradation of the ETHYLENE INSENSITIVE3 (EIN3) transcription factor. Here, we report that the level of EIL1 protein is upregulated by ethylene through an EBF1/2-dependent pathway. Genetic analysis revealed that EIL1 and EIN3 cooperatively but differentially regulate a wide array of ethylene responses, with EIL1 mainly inhibiting leaf expansion and stem elongation in adult plants and EIN3 largely regulating a multitude of ethylene responses in seedlings. When EBF1 and EBF2 are disrupted, EIL1 and EIN3 constitutively accumulate in the nucleus and remain unresponsive to exogenous ethylene application. Further study revealed that the levels of EBF1 and EBF2 proteins are downregulated by ethylene and upregulated by silver ion and MG132, suggesting that ethylene stabilizes EIN3/EIL1 by promoting EBF1 and EBF2 proteasomal degradation. Also, we found that EIN2 is indispensable for mediating ethylene-induced EIN3/EIL1 accumulation and EBF1/2 degradation, whereas MKK9 is not required for ethylene signal transduction, contrary to a previous report. Together, our studies demonstrate that ethylene similarly regulates EIN3 and EIL1, the two master transcription factors coordinating myriad ethylene responses, and clarify that EIN2 but not MKK9 is required for ethylene-induced EIN3/EIL1 stabilization. Our results also reveal that EBF1 and EBF2 act as essential ethylene signal transducers that by themselves are subject to proteasomal degradation.read more
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Hormone Crosstalk in Plant Disease and Defense: More Than Just JASMONATE-SALICYLATE Antagonism
TL;DR: Recent advances are reported, updating current knowledge on classical defense hormones SA, JA, and ET, and the roles of auxin, abscisic acid (ABA), cytokinins (CKs), and brassinosteroids in molding plant-pathogen interactions are updated.
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MAPK cascades in plant disease resistance signaling.
Xiangzong Meng,Shuqun Zhang +1 more
TL;DR: Recent advances in the identification of upstream receptors/sensors and downstream MAPK substrates revealed the molecular mechanisms underlying MAPK functions in plant disease resistance and emerged as battlegrounds of plant-pathogen interactions.
Journal ArticleDOI
Derepression of ethylene-stabilized transcription factors (EIN3/EIL1) mediates jasmonate and ethylene signaling synergy in Arabidopsis
Ziqiang Zhu,Fengying An,Ying Feng,Pengpeng Li,L. Xue,Mu A,Zhiqiang Jiang,Jong-Myong Kim,Taiko Kim To,Wei Li,Xinyan Zhang,Qiang Yu,Zhi Dong,Wen-Qian Chen,Motoaki Seki,Jianmin Zhou,Hongwei Guo +16 more
TL;DR: EIN3/EIL1 is identified as a key integration node whose activation requires both JA and ET signaling, and transcriptional derepression is illustrated as a common mechanism to integrate diverse signaling pathways in the regulation of plant development and defense.
Journal ArticleDOI
Ethylene Role in Plant Growth, Development and Senescence: Interaction with Other Phytohormones
Noushina Iqbal,Nafees A. Khan,Antonio Ferrante,Alice Trivellini,Alessandra Francini,M. I. R. Khan +5 more
TL;DR: The present review focused on ethylene’s role in the development and senescence processes in leaves, flowers and fruits, paying special attention to the complex networks of ethylene crosstalk with other hormones.
Journal ArticleDOI
Ethylene Signaling Negatively Regulates Freezing Tolerance by Repressing Expression of CBF and Type-A ARR Genes in Arabidopsis
TL;DR: This study demonstrates that ethylene negatively regulates cold signaling at least partially through the direct transcriptional control of cold-regulated CBFs and type-A ARR genes by EIN3, and provides evidence that type- A ARRs function as key nodes to integrate ethylene and cytokinin signaling in regulation of plant responses to environmental stress.
References
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Journal ArticleDOI
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Jose M. Alonso,Anna Stepanova,Thomas J. Leisse,Christopher J. Kim,Huaming Chen,Paul Shinn,Denise K. Stevenson,Justin Zimmerman,Pascual Barajas,Rosa Cheuk,Carmelita Gadrinab,Collen Heller,Albert Jeske,Eric Koesema,Cristina C. Meyers,Holly Parker,Lance Prednis,Yasser Ansari,Nathan Choy,Hashim Deen,Michael Geralt,Nisha Hazari,Emily Hom,Meagan Karnes,Celene Mulholland,Ral Ndubaku,Ian Thomas Schmidt,Plinio Guzmán,Laura Aguilar-Henonin,Markus Schmid,Detlef Weigel,David E. Carter,Trudy Marchand,Eddy Risseeuw,Debra Brogden,Albana Zeko,William L. Crosby,Charles C. Berry,Joseph R. Ecker +38 more
TL;DR: Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels, and insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.
Book
Ethylene in plant biology
TL;DR: This book discusses Ethylene Analysis and Properties of the Gas, the Role of Ethylene in Agriculture, and Roles and Physiological Effects ofEthylene in Plant Physiology: Dormancy, Growth and Development.
Ethylene in Plant Biology
TL;DR: This book discusses Ethylene Analysis and Properties of the Gas, the Role of Ethylene in Agriculture, and Roles and Physiological Effects ofEthylene in Plant Physiology: Dormancy, Growth and Development.
Journal ArticleDOI
CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases
TL;DR: A recessive Arabidopsis mutant, ctr1, that constitutively exhibits seedling and adult phenotypes observed in plants treated with the plant hormone ethylene is isolated and the DNA sequences of four mutant alleles were determined.
Journal ArticleDOI
Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators
TL;DR: An early step in ethylene signal transduction in plants may involve transfer of phosphate as in prokaryotic two-component systems.
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