Journal ArticleDOI
Evaluation of the relative efficacy of various techniques for deproteinizing plasma samples prior to high-performance liquid chromatographic analysis
TLDR
A number of other potentially useful methods of protein removal are described using the much moresensitive Lowry method of protein determination to evaluate their efficiency.About:
This article is published in Journal of Chromatography B: Biomedical Sciences and Applications.The article was published on 1981-12-11. It has received 214 citations till now.read more
Citations
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Journal ArticleDOI
Spectrophotometric Determination of Serum Nitrite and Nitrate by Copper–Cadmium Alloy
TL;DR: The present method provides a simple, cost-effective assay for the estimation of stable oxidation products of nitric oxide in biological samples.
Journal ArticleDOI
Potent and Selective Inhibition of Human Cytomegalovirus Replication by 1263W94, a Benzimidazole l-Riboside with a Unique Mode of Action
Karen K. Biron,Robert J. Harvey,Stanley C. Chamberlain,Steven S. Good,Albert A. Smith,Michelle G. Davis,Christine L. Talarico,Wayne H. Miller,Robert G. Ferris,R E Dornsife,Sylvia C. Stanat,John C. Drach,Leroy B. Townsend,George W. Koszalka +13 more
TL;DR: The mechanism of the antiviral effect of l-riboside 1263W94 is thus distinct from those of GCV and of BDCRB, including those resistant to ganciclovir, foscarnet, andBDCRB.
Journal ArticleDOI
Memrane extraction in analytical chemistry
TL;DR: The principles and application of porous and non-porous membrane extraction techniques for sample preparation (pretreatment) in analytical chemistry are reviewed, with emphasis on aqueous samples of biological, environmental, or food origin this paper.
Journal ArticleDOI
Sample preparation for biomedical analysis.
TL;DR: A review of sample preparation techniques for biomedical analysis is presented in this paper, which can be classified into four groups: release of the analyte from the matrix, removal of endogenous material, liquid handling procedures and the enhancement of selectivity and sensitivity.
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Simultaneous determination of 6 beta-blockers, 3 calcium-channel antagonists, 4 angiotensin-II antagonists and 1 antiarrhytmic drug in post-mortem whole blood by automated solid phase extraction and liquid chromatography mass spectrometry: Method development and robustness testing by experimental design
Lena Kristoffersen,Elisabeth Leere Øiestad,Mimi Stokke Opdal,Mette Krogh,Elsa Lundanes,Asbjørg S. Christophersen +5 more
TL;DR: The method is used for determination of cardiovascular drugs in post-mortem whole blood samples from forensic autopsy cases and robustness tested by systematically searching for satisfactory conditions using experimental designs including factorial and response surface designs.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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A method for the preparation of blood filtrates for the determination of sugar
Journal ArticleDOI
Ultrafiltration in Serum Protein Binding Determinations
TL;DR: In this paper, it was shown that the ultrafiltrate concentration remains constant during filtration, and thus binding equilibria are not disturbed by this procedure, suggesting that an arbitrary restriction on the volume filtered is unnecessary.
Journal ArticleDOI
Ultrafiltration is theoretically equivalent to equilibrium dialysis but much simpler to carry out.
Judith A. Sophianopoulos,Samuel J. Durham,Alkis J. Sophianopoulos,Harvey L. Ragsdale,Wendell P. Cropper +4 more
TL;DR: It is shown that if one component of a reaction at equilibrium is freely diffusible through a semipermeable membrane, and if an aliquot of this component is removed through the membrane at its equilibrium concentration, the concentration of this components in the reaction mixture remains unchanged.