Journal ArticleDOI
Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter
TLDR
This study demonstrates that the PFLD1 promoter is at least as efficient as the PAOX1 for extracellular expression of heterologous proteins in P. pastoris bioreactor cultures and provides a first basis for the further design of methanol-free high cell density fed-batch cultivation strategies for controlled overproduction of foreign proteins in the organism.About:
This article is published in Journal of Biotechnology.The article was published on 2004-04-08. It has received 108 citations till now. The article focuses on the topics: Pichia pastoris & Rhizopus oryzae.read more
Citations
More filters
Journal ArticleDOI
Heterologous protein production using the Pichia pastoris expression system.
TL;DR: The Pichia pastoris expression system is being used successfully for the production of various recombinant heterologous proteins and the importance of optimizing the physicochemical environment for efficient and maximal recombinant protein production in bioreactors and the role of process control in optimizing protein production is reviewed.
Book ChapterDOI
Recombinant Protein Production in Yeasts
TL;DR: Advantages and limitations of the main and most promising yeast hosts, including Saccharomyces cerevisiae, Pichia pastoris, and Hansenula polymorpha as those presently used in large scale production of heterologous proteins are summarized.
Journal ArticleDOI
Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review
TL;DR: The methylotrophic yeast Pichia pastoris has been widely reported as a suitable expression system for heterologous protein production and the use of mixed substrates, on-line monitoring of the key fermentation parameters (methanol) and control algorithms applied to the bioprocess are reviewed and discussed in detail.
Journal ArticleDOI
Recombinant protein production in yeasts.
TL;DR: This review summary summarizes advantages and limitations of the main and most promising yeast hosts for the production of heterologous proteins inRecombinant DNA technologies.
Journal ArticleDOI
Regulation of methanol utilisation pathway genes in yeasts
TL;DR: In this paper, the role of cis and trans acting factors in the expression of methanol utilisation pathway genes is reviewed both in the context of the native cell environment as well as in heterologous hosts.
References
More filters
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.
TL;DR: Gene splicing by overlap extension is a new approach for recombining DNA molecules at precise junctions irrespective of nucleotide sequences at the recombination site and without the use of restriction endonucleases or ligase.
Journal ArticleDOI
Heterologous protein expression in the methylotrophic yeast Pichia pastoris
TL;DR: This paper reviews the P. pastoris expression system: how it was developed, how it works, and what proteins have been produced and describes new promoters and auxotrophic marker/host strain combinations which extend the usefulness of the system.
Journal ArticleDOI
Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter.
TL;DR: In this paper, the authors reported the cloning and sequence of the glyceraldehyde-3-phosphate dehydrogenase gene (GAP) from the yeast Pichia pastoris.
Journal ArticleDOI
Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris
TL;DR: Two DNA fragments containing putative control regions regulating the expression of the alcohol oxidase and dihydroxy-acetone synthase genes from the methylotrophic yeast Pichia pastoris were used in the construction of vectors for theexpression of the Escherichia coli lacZ gene.