Journal ArticleDOI
Expression of a truncated hepatitis E virus capsid protein in the protozoan organism Leishmania tarentolae and its application in a serological assay.
TLDR
It was shown that L. tarentolae exhibits a remarkable alternative expression strategy for viral antigens with considerable advantages of a eukaryotic protein expression host.About:
This article is published in Journal of Virological Methods.The article was published on 2013-10-01. It has received 15 citations till now. The article focuses on the topics: Capsid & Recombinant DNA.read more
Citations
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Journal ArticleDOI
Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules
Isabelle Legastelois,Sophie Buffin,Isabelle Peubez,Charlotte Mignon,Régis Sodoyer,Bettina Werle +5 more
TL;DR: This review provides an overview of the evolution of traditional expression systems, including mammalian cells, yeast and E.coli, but also alternative systems such as other bacteria than E. coli, transgenic animals, insect cells, plants and microalgae, Tetrahymena thermophila, Leishmania tarentolae, filamentous fungi, cell free systems, and the incorporation of non-natural amino acids.
Journal ArticleDOI
Indirect ELISA based on Hendra and Nipah virus proteins for the detection of henipavirus specific antibodies in pigs
Kerstin Fischer,Sandra Diederich,Greg Smith,Sven Reiche,Vinicius Pinho dos Reis,Eileen Stroh,Martin H. Groschup,Hana M. Weingartl,Anne Balkema-Buschmann +8 more
TL;DR: Enzyme-linked Immunosorbent Assays (ELISA) were developed for the detection of HeV or NiV specific antibodies in porcine serum samples and enabled the differentiation between HeV and NiV infections, since as expected, the sera displayed higher reactivity with the respective homologous antigens.
Journal ArticleDOI
Detection of Serum Antibodies to Hepatitis E Virus Based on HEV Genotype 3 ORF2 Capsid Protein Expressed in Nicotiana benthamiana.
Milena Mazalovska,Nikola Varadinov,Tsvetoslav Koynarski,Ivan Minkov,Pavel Teoharov,George P. Lomonossoff,Gergana Zahmanova +6 more
TL;DR: A method for the production of HEV ORF2 protein in N. benthamiana and the usefulness of this protein for the serological detection of anti-HEV antibodies in both humans and swine is described.
Journal ArticleDOI
A Non-pathogenic Recombinant Leishmania Expressing Lipophosphoglycan 3 Against Experimental Infection with Leishmania infantum.
Leila Pirdel,Safar Farajnia +1 more
TL;DR: Results indicated that the administration of live recombinant Leishmania produced a significant high level of IFN‐γ accompanied by reduced levels of IL‐10 as compared to wild‐type parasites as live vaccine control, thus suggesting the induction of a Th1‐type immune response in a mouse model of visceral leishmaniasis.
Journal ArticleDOI
Expression, characterisation and antigenicity of a truncated Hendra virus attachment protein expressed in the protozoan host Leishmania tarentolae.
Kerstin Fischer,Vinicius Pinho dos Reis,Stefan Finke,Lucie Sauerhering,Eileen Stroh,Axel Karger,Andrea Maisner,Martin H. Groschup,Sandra Diederich,Anne Balkema-Buschmann +9 more
TL;DR: The purification of a truncated, Strep-tag labelled and soluble version of the HeV attachment protein (sHeV G) expressed in stably transfected L. tarentolae cells supports the preserved antigenicity of the purified protein.
References
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Journal ArticleDOI
Hepatitis E virus and chronic hepatitis in organ-transplant recipients.
Nassim Kamar,Janick Selves,Jean-Michel Mansuy,Leila Ouezzani,Jean-Marie Péron,Joelle Guitard,Olivier Cointault,Laure Esposito,Florence Abravanel,Marie Danjoux,Dominique Durand,Jean-Pierre Vinel,Jacques Izopet,Lionel Rostaing +13 more
TL;DR: The time from transplantation to diagnosis was significantly shorter and the total counts of lymphocytes and of CD2, CD3, and CD4 T cells were significantly lower in patients in whom chronic disease developed.
Journal ArticleDOI
Solubilization and refolding of bacterial inclusion body proteins
TL;DR: Analysis of the dominant forces causing aggregation during inclusion body formation provides information to develop suitable mild solubilization procedures for inclusion body proteins and restoration of native-like secondary structure is assumed.
Journal ArticleDOI
Pig liver sausage as a source of hepatitis E virus transmission to humans.
Philippe Colson,Patrick Borentain,Benjamin Queyriaux,Benjamin Queyriaux,Mamadou Kaba,Valérie Moal,Pierre Gallian,Laurent Heyries,Didier Raoult,René Gerolami +9 more
TL;DR: The findings strongly support the hypothesis of HEV infection through ingestion of raw figatellu, a traditional pig liver sausage widely eaten in France and commonly consumed raw, as a source ofHEV infection.
Journal ArticleDOI
Electroporation in ‘intracellular’ buffer increases cell survival
Journal ArticleDOI
Zoonotic hepatitis E: animal reservoirs and emerging risks.
TL;DR: Several lines of evidence indicate that, in some cases involving HEV genotypes 3 and 4, animal to human transmissions occur, and individuals with direct contact with animals are at higher risk of HEV infection.