Expression of Sox9, Mis, and Dmrt1 in the gonad of a species with temperature‐dependent sex determination
TLDR
It is found the first evidence that Sox9 expression is preferentially organized in the testis early in the temperature‐sensitive period in a species with temperature‐dependent sex determination (Trachemys scripta).Abstract:
Sex determination in vertebrates, the process of forming an ovary or testis from a bipotential gonad, can be initiated by genetic or environmental factors. Elements of the downstream molecular pathways underlying these different sex-determining mechanisms have been evolutionarily conserved. We find the first evidence that Sox9 expression is preferentially organized in the testis early in the temperature-sensitive period in a species with temperature-dependent sex determination (Trachemys scripta). This pattern occurs before sexually dimorphic Mis expression and in a temporal hierarchy that is similar to mammals. Furthermore, we extend previous findings that Dmrt1 expression at early stages of sex determination has a dimorphic pattern consistent with a possible upstream role in determining the fate of the bipotential gonad.read more
Citations
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Journal ArticleDOI
Sex Determination: Why So Many Ways of Doing It?
Doris Bachtrog,Judith E. Mank,Catherine L. Peichel,Mark Kirkpatrick,Sarah P. Otto,Tia-Lynn Ashman,Matthew W. Hahn,Jun Kitano,Itay Mayrose,Ray Ming,Nicolas Perrin,Laura Ross,Nicole Valenzuela,Jana C. Vamosi +13 more
TL;DR: The current understanding of how and why sex determination evolves in animals and plants is reviewed.
Journal ArticleDOI
The avian Z-linked gene DMRT1 is required for male sex determination in the chicken
Craig A. Smith,Kelly N. Roeszler,Thomas Ohnesorg,David M. Cummins,Peter G. Farlie,Timothy J. Doran,Andrew H. Sinclair +6 more
TL;DR: RNA interference is used to knock down DMRT1 in early chicken embryos to support the Z dosage hypothesis for avian sex determination, and results indicate that D MRT1 is required for testis determination in the chicken.
Journal ArticleDOI
Sexual Dimorphic Expression of Genes in Gonads During Early Differentiation of a Teleost Fish, the Nile Tilapia Oreochromis niloticus
Shigeho Ijiri,Hiroyo Kaneko,Tohru Kobayashi,Deshou Wang,Deshou Wang,Fumie Sakai,Bindhu Paul-Prasanth,Masaru Nakamura,Yoshitaka Nagahama +8 more
TL;DR: It is concluded that the sex-specific expression of foxl2 and cyp19a1a in XX gonads and dmrt1 in XY gonads during early gonadal differentiation is critical for undifferentiated gonads to differentiate into either the ovary or testis in the Nile tilapia.
Journal ArticleDOI
Environmental effects on fish sex determination and differentiation.
TL;DR: In tilapia and sea bass, domestic stocks and field-collected populations showed similar patterns of thermosensitivity under controlled conditions, and transitional forms within a genetic sex determination (GSD) and environmental sexdetermination (ESD) continuum seem to exist.
Journal ArticleDOI
Dmrt genes in the development and evolution of sexual dimorphism.
TL;DR: A number of common themes in the sexual development of different taxa are highlighted, how Dmrt genes have acquired new roles during animal evolution is discussed, and how they have contributed to the origin of novel sex-specific traits are shown.
References
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Jamie W. Foster,M A Dominguez-Steglich,S Guioli,Cheni Kwok,Polly A. Weller,Milena Stevanovic,J Weissenbach,S Mansour,I D Young,Peter N. Goodfellow +9 more
TL;DR: Cloning of a translocation chromosome breakpoint from a sex-reversed patient with campomelic dysplasia, followed by mutation analysis of an adjacent gene, indicates that SOX9, an SRY-related gene, is involved in both bone formation and control of testis development.
Journal Article
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DMY is a Y-specific DM-domain gene required for male development in the medaka fish
Masaru Matsuda,Yoshitaka Nagahama,Ai Shinomiya,Tadashi Sato,Chika Matsuda,Tohru Kobayashi,Craig E. Morrey,Naoki Shibata,Shuichi Asakawa,Nobuyoshi Shimizu,Hiroshi Hori,Satoshi Hamaguchi,Mitsuru Sakaizumi +12 more
TL;DR: Rec recombinant breakpoint analysis is used to restrict the sex-determining region in medaka fish to a 530-kilobase stretch of the Y chromosome, and it is suggested that thesex-specific DMY is required for testicular development and is a prime candidate for the medaka sex-Determining gene.
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TL;DR: It will be shown that the calculation of Mean Normalized Expressions has to be used for processing simplex PCR data, while multiplex PCRData should preferably be processed by calculating Normalizedexpressions.
Journal ArticleDOI
Sox9 induces testis development in XX transgenic mice.
TL;DR: It is shown that Sox9 is sufficient to induce testis formation in mice, indicating that it can substitute for the sex-determining gene Sry, which is associated with male-to-female sex reversal in humans.