Journal ArticleDOI
Extraction of extracellular polymeric substances (EPS) from biofilms using a cation exchange resin
Andreas Jahn,Per Halkjær Nielsen +1 more
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TLDR
Proteins and humic substances can be extracted from biofilm EPS in considerable amounts and should be investigated more in detail when analysing the properties of the EPS matrix of a biofilm.About:
This article is published in Water Science and Technology.The article was published on 1995-01-01. It has received 108 citations till now. The article focuses on the topics: Extraction (chemistry) & Extracellular polymeric substance.read more
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Journal ArticleDOI
Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review.
TL;DR: As EPS are very complex, the knowledge regarding EPS is far from complete and much work is still required to fully understand their precise roles in the biological treatment process.
Journal ArticleDOI
A unified theory for extracellular polymeric substances, soluble microbial products, and active and inert biomass.
TL;DR: A unified theory shows that the apparently distinct products from the SMP and EPS schools overlap each other, and shows that Soluble EPS is actually SMP, or the sum of UAP and BAP.
Journal ArticleDOI
Surface properties of sludge and their role in bioflocculation and settleability
Bao-Qiang Liao,D.G. Allen,Ian G. Droppo,Gary G. Leppard,Gary G. Leppard,Steven N. Liss,Steven N. Liss +6 more
TL;DR: The results indicate that it is the surface properties, hydrophobicity, surface charge and composition of EPS, of sludge, rather than the quantity of EPS that govern bioflocculation, and in contrast, the EPS content is more important in controlling the settleability of sludges.
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Composition and Distribution of Extracellular Polymeric Substances in Aerobic Flocs and Granular Sludge
TL;DR: The comparison of EPS extraction methods explains how significant cell lysis and contamination by dead biomass leads to different and opposing conclusions and indicates that granule formation and stability are dependent on a noncellular, protein core.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Extraction of extracellular polymers from activated sludge using a cation exchange resin
TL;DR: In this article, the extraction of water soluble extracellular polymeric substances (EPS) from activated sludge was investigated, which consisted mainly of protein but also humic compounds, carbohydrates, uronic acids and DNA.
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Assay for nanogram quantities of DNA in cellular homogenates
TL;DR: The DNA concentration of a crude cellular homogenate can be measured accurately in the nanogram range using the fluorescence enhancement of 4′,6-diamidino-2-phenylindole (DAPI) or bisbenzimidazole (Hoechst H 33258) complexed with DNA.
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Carbohydrate Interference and Its Correction in Pectin Analysis Using the m-Hydroxydiphenyl Method
TL;DR: The colorimetric assay using m-hydroxydiphenyl for analysis of uronic acids is used to measure pectins in plant materials because of its relative insensitivity to the presence of nonuronide carbohydrates as mentioned in this paper.
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Polysaccharide Antigens of Pseudomonas Aeruginosa
TL;DR: The major polysaccharide antigens of P. aeruginosa are the cell-wall lipopolysaccharides many of which have an acidic poly saccharide chain (O-antigen) rich in unusual amino sugars and the D-rhamnose-rich poly Saccharide antigen common to many serologically distinct strains is also associated with the lipopolySaccharide.