Journal ArticleDOI
Extrusion and rotation of the central‐pair microtubules in detergent‐treated chlamydomonas flagella
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This article is published in Progress in Clinical and Biological Research.The article was published on 1982-01-01. It has received 73 citations till now. The article focuses on the topics: Chlamydomonas.read more
Citations
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Journal ArticleDOI
Submicromolar levels of calcium control the balance of beating between the two flagella in demembranated models of Chlamydomonas.
Ritsu Kamiya,George B. Witman +1 more
TL;DR: The results show that there are functional differences between the two axonemes of Chlamydomonas, and the differential responses of the ax onemes to submicromolar concentrations of Ca++ may form the basis for phototactic turning.
Journal ArticleDOI
The radial spokes and central apparatus: mechano-chemical transducers that regulate flagellar motility.
Elizabeth F. Smith,Pinfen Yang +1 more
TL;DR: A model in which the central apparatus and radial spokes serve as mechano-chemical sensors to control motility in 9+2 cilia and flagella is proposed.
Journal ArticleDOI
Substructure of inner dynein arms, radial spokes, and the central pair/projection complex of cilia and flagella.
Ursula Goodenough,John E. Heuser +1 more
TL;DR: The substructure of the components of the axoneme interior--the inner dynein arms, the radial spokes, and the central pair/projection complex- -was analyzed for Chlamydomonas, Tetrahymena, Strongelocentrotus, and Mnemiopsis using the quick-freeze, deep-etch technique.
Journal ArticleDOI
Rotation and translocation of microtubules in vitro induced by dyneins from Tetrahymena cilia
Ronald D. Vale,Yoko Y. Toyoshima +1 more
TL;DR: A quantitative in vitro motility assay in which dynein adsorbed onto a glass surface induces linear translocation of purified bovine microtubules and shows that both 22S and 14S dyneins from Tetrahymena cilia induce movement but have distinct motile properties.
Journal ArticleDOI
Radial spoke proteins of Chlamydomonas flagella
Pinfen Yang,Dennis R. Diener,Chun Yang,Takahiro Kohno,Gregory J. Pazour,Jennifer M. Dienes,Nathan S. Agrin,Stephen M. King,Winfield S. Sale,Ritsu Kamiya,Joel L. Rosenbaum,George B. Witman +11 more
TL;DR: Mass spectrometry is used to identify 10 additional radial spoke proteins, including the recently described HSP40 family member and a second spoke stalk protein predicted to be a molecular chaperone, implying that there is a sophisticated mechanism for the assembly of this large complex.
References
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Journal ArticleDOI
Flagellar motion and fine structure of the flagellar apparatus in chlamydomonas
TL;DR: The biflagellate alga Chlamydomonas reinhardi was studied with the light and electron microscopes to determine the behavior of flagella in the living cell and the structure of the basal apparatus of the flagellar apparatus.
Journal ArticleDOI
Nutritional studies with chlamydomonas reinhardi
Ruth Sager,S. Granick +1 more
TL;DR: The nutritional studies to be reported here are concerned, first, with vegetative growth of the organism in light and dark and, second, with the nutritional conditions which the authors have found to be of critical importance in controlling the sexual phases of the life-cycle.
Journal ArticleDOI
Isolated flagellar apparatus of Chlamydomonas: characterization of forward swimming and alteration of waveform and reversal of motion by calcium ions in vitro.
J.S. Hyams,Gary G. Borisy +1 more
TL;DR: Isolated flagellar apparatus could be induced to change from forward to reverse motion in vitro by manipulation of exogenous calcium ions, and the 2 types of motion were directly comparable to recorded responses of living cells.
Journal ArticleDOI
Calcium control of waveform in isolated flagellar axonemes of chlamydomonas
TL;DR: Results indicate that free Ca(++) binds directly to an axonemal component to alter flagellar waveform, and that neither the 3.0S CaATPase nor the basal bodies are directly involved in this change.
Journal ArticleDOI
Rotation and twist of the central-pair microtubules in the cilia of Paramecium.
C K Omoto,C Kung +1 more
TL;DR: Both serial thin- section and thick-section high-voltage electron microscopy show that whenever a twist in the central pair is seen, it is always left-handed, consistent with the hypothesis that the central couple continuously rotates counterclockwise with the rotation originating at the base of the cilium.
Related Papers (5)
Chlamydomonas flagellar mutants lacking radial spokes and central tubules. Structure, composition, and function of specific axonemal components.
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Adenosine triphosphate-induced sliding of tubules in trypsin-treated flagella of sea-urchin sperm.
Keith E. Summers,I. R. Gibbons +1 more