Journal ArticleDOI
Fate of bacterial plasmid DNA during uptake by barley protoplasts
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TLDR
It seemed that covalently closed circular (CCC) duplex plasmid DNA might be more resistant to depolymerization, and also that it might eventually allow insertion of desirable genes into protoplasts since techniques for gene insertion into plasmids have been developed.About:
This article is published in FEBS Letters.The article was published on 1977-07-01. It has received 26 citations till now. The article focuses on the topics: Plasmid preparation & Transfer DNA.read more
Citations
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Patent
Transformation of hereditary material of plants
Jerzy Paszkowski,Ingo Potrykus,Barbara Hohn,Raymond D. Shillito,Thomas Hohn,Michael W. Saul,Vaclav Mandak +6 more
TL;DR: In this paper, a novel process for the direct transfer of foreign genes to plant genomes is described, which comprises placing a gene under the control of plant expression signals and transferring it, by contact with protoplasts without the aid of natural systems for infecting plants, direct to plant cells from which genetically transformed plants can subsequently be derived.
Journal ArticleDOI
Attempted pollen-mediated plant transformation employing genomic donor DNA.
TL;DR: Experiments were conducted to test the validity of previous reports of pollen-mediated plant transformation utilizing genomic donor DNA, and using well-defined multiple markers, it is unable to confirm the earlier claims of high efficiency pollen- mediated transformation employing genomic donorDNA.
Book ChapterDOI
Liposomes: the development of a new carrier system for introducing nucleic acid into plant and animal cells.
Journal ArticleDOI
The permeability of electroporated cells and protoplasts of sugar beet.
TL;DR: Results of timecourse studies of phenosafranine accumulation and retention indicated that pores are formed or enlarged rapidly in the plasmamembrane and remain permeable to phenosAFranine for relatively long periods; the half-life of the ‘pores’ was temperaturedependent.
Journal ArticleDOI
Incorporation and maintenance of recombinant-DNA plasmid vehicles pBR313 and pCR1 in plant protoplasts
TL;DR: Evidence is provided for the stable maintenance of genecloning plasmids in protoplasts of carrots, cowpea, periwinkle and turnips, and provide conditions most conductive for plasmid uptake by these plant cells.
References
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Journal ArticleDOI
A simple method for the preparation of large quantities of pure plasmid DNA.
TL;DR: Polyethylene glycol quantitatively precipitates plasmid DNA of molecular weight 6-123-10-6 from cleared lysates ofplasmid-carrying bacterial strains, unchanged in length and in transformation efficiency for Escherichia coli K12.
Journal ArticleDOI
Regeneration of whole plants from isolated mesophyll protoplasts of tobacco
TL;DR: Theoret. Leningrad un.-ta Mr. Fischereiwiss (zitiert nach Woyn~rovich 1963).
Journal ArticleDOI
Replication and transcription of eukaryotic DNA in Escherichia coli.
John Morrow,Stanley N. Cohen,Annie C. Y. Chang,Herbert W. Boyer,Howard M. Goodman,Robert B. Helling +5 more
TL;DR: Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation.
Journal ArticleDOI
Manipulation of restriction targets in phage λ to form receptor chromosomes for DNA fragments
Noreen E. Murray,Kenneth Murray +1 more
TL;DR: Fragments of DNA from derivatives of phage λ having either one or two targets for R.EcoRI have been joined in new combinations to give biologically active phage genomes.
Journal ArticleDOI
Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA.
TL;DR: A bacteriophage lambda strain has been constructed and a method developed by which DNA from potentially any source can be covalently inserted through EcoRI cohesive ends into the middle of the lambda DNA.
Related Papers (5)
Escherichia coli plasmid pBR313 insertion into plant protoplasts and into their nuclei.
Paul F. Lurquin,Clarence I. Kado +1 more
Uptake of Single-Stranded Bacteriophage DNA by Isolated Tobacco Protoplasts
Masahiko Suzuki,Itaru Takebe +1 more