Journal ArticleDOI
Fiber-Optic Probe for Collection of Two-Photon Excited Fluorescence Spectra in Low-Temperature Glassy Solvents
Debora M. Dinkel,Fred E. Lytle +1 more
TLDR
In this article, a fiber-optic probe directly frozen in the glassy sample allowed two-photon excited fluorescence spectra to be obtained, which is stable because the fiber restricts beam-induced refractive index changes to harmless expansion.Abstract:
Low-repetition-rate pulsed lasers are excellent sources for two-photon excitation of samples frozen in low-temperature glassy solvents. Unfortunately, similar efforts to combine synchronously-pumped dye laser excitation with normal cryostats and sample cells have consistently failed. The continuous nature of the laser creates a weak refractive index gradient which causes the beam to wander randomly in space. We have found that a fiber-optic probe directly frozen in the glassy sample permits two-photon excited fluorescence spectra to be obtained. This arrangement is stable because the fiber restricts beam-induced refractive index changes to harmless expansion. Studies of fluxional fluorophores indicate that the viscosity at the fiber tip is not significantly changed under conditions of average power known to cause beam wander in ordinary cryostat cells.read more
Citations
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Book ChapterDOI
Low-Temperature Spectroscopy
TL;DR: The chapter provides a comprehensive review of all low-temperature studies on biological systems and goes beyond the traditional use of low temperatures to trap intermediate chemical states and to speculate the insight that low-Temperature spectroscopy provides the role of protein dynamics in controlling chemical reactions.
Journal ArticleDOI
Design of a Simple Cryogenic System for Ultraviolet-Visible Absorption Spectroscopy with a Back-Reflectance Fiber Optic Probe
TL;DR: A convenient and inexpensive technique for the rapid acquisition of absorption spectra from small samples at cryogenic temperatures using a home built cryostat with novel collection optics, found to be proportional to concentration, displaying Beer's law–like behavior.
References
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Book
Amorphous solids : low-temperature properties
TL;DR: In this article, the authors proposed a tunneling model based on the Vibrational Density of States of Amorphous Semiconductors (VDS) and three-pulse electric echo.
Journal ArticleDOI
Die Viskositätsabhängigkeit der Fluoreszenzquantenausbeuten einiger Farbstoffsysteme
Th. Förster,G. Hoffmann +1 more
TL;DR: Oster et al. as mentioned in this paper untersuchten an dem Diphenylmethanfarbstoff Auramin 0 the Zunahme der Pluoreszenzausbeute with der Viskosität in Mischungen von Wasser, Glycerin, and Glukose verschiedener Zusammensetzungen and Temperaturen.
Journal ArticleDOI
Torsional dynamics of molecules on barrierless potentials in liquids. I. Temperature and wavelength dependent picosecond studies of triphenyl‐methane dyes
Dor Ben-Amotz,Charles B. Harris +1 more
TL;DR: In this paper, the nonradiative decay dynamics of crystal violet, and other triphenylmethane dyes, dissolved in a variety of solvents, are studied as a function of temperature.
Journal ArticleDOI
Effects of solvent on TMP photophysics. Transition from no barrier to barrier case, induced by solvent properties
Villy Sundström,Tomas Gillbro +1 more
TL;DR: In this paper, the authors studied the radiationless relaxation of triphenylmethane (TPM) molecules in the n-alcohols methanol to octadecanol with picosecond absorption recovery techniques as a function of viscosity, temperature and wavelength of the exciting and analyzing light.
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