Journal ArticleDOI
Fingerprint matching of E. coli strains with matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry of whole cells using a modified correlation approach
Randy J. Arnold,James P. Reilly +1 more
TLDR
A mathematical algorithm to compare and distinguish matrix-assisted laser desorption/ionization mass spectra of whole bacteria cells is developed and it has distinguished twenty five different strains of a single bacteria species, E. coli.Abstract:
We have developed a mathematical algorithm to compare and distinguish matrix-assisted laser desorption/ionization (MALDI) mass spectra of whole bacteria cells. This fingerprint matching technique eliminates the subjectivity involved in visually comparing two spectra to determine whether they match and it provides a quantitative measure of spectral similarity. Using it, we have distinguished twenty five different strains of a single bacteria species, E. coli. Cells are grown in culture, samples are prepared, and MALDI-TOF mass spectra are recorded for each strain. Pairs of spectra are compared by a modified cross-correlation procedure. This modified approach increases the sensitivity of correlation analysis to small spectral differences. The technique can be fine-tuned by varying the number of intervals into which spectra are divided.read more
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Journal ArticleDOI
Characterization of intact microorganisms by MALDI mass spectrometry.
TL;DR: The application of MALDI mass spectrometry to desorb protein biomarkers from intact viruses, bacteria, fungus, and spores is the focus of this review.
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SERS Nanoparticles in Medicine: From Label-Free Detection to Spectroscopic Tagging.
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MALDI-TOF mass spectrometry of bacteria.
TL;DR: The demonstrated capability of taxonomic classification at the strain level, using unprocessed cells, opens the possibility that MALDI-TOF and similar mass spectrometry approaches may contribute significantly to fulfilling emerging needs for the development of near real-time methods for the characterization of bacteria.
Journal ArticleDOI
Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Fast and Reliable Identification of Clinical Yeast Isolates
G. Marklein,Michaele Josten,Ursula Klanke,E. Müller,R. Horré,T. Maier,T. Wenzel,Markus Kostrzewa,Gabriele Bierbaum,Achim Hoerauf,Hans-Georg Sahl +10 more
TL;DR: Overall, MALDI-TOF MS proved a most rapid and reliable tool for the identification of yeasts and yeast-like fungi, with the method providing a combination of the lowest expenditure of consumables, easy interpretation of results, and a fast turnaround time.
Journal ArticleDOI
Mass spectrometry tools for the classification and identification of bacteria
Sascha Sauer,Magdalena Kliem +1 more
TL;DR: Recently, several mass spectrometry methods for the classification and identification of bacteria and other microorganisms, as well as new software analysis tools, have been developed and their potential for transfer into microbiology laboratories are discussed.