Journal ArticleDOI
Form and function of retroviral proviruses
TLDR
Retroviruses have proved to be useful reagents for studying genetic and epigenetic changes in eukaryotic cells, and their capacity to establish a DNA (proviral) form of their RNA genomes as a stable component of host chromosomes, in either somatic or germinal cells.Abstract:
Retroviruses have proved to be useful reagents for studying genetic and epigenetic (such as regulatory) changes in eukaryotic cells, for assessing functional and structural relationships between transposable genetic elements, for inducing insertional mutations, including some important in oncogenesis, and for transporting genes into eukaryotic cells, either after natural transduction of putative cellular oncogenes or after experimental construction of recombinant viruses. Many of these properties of retroviruses depend on their capacity to establish a DNA (proviral) form of their RNA genomes as a stable component of host chromosomes, in either somatic or germinal cells.read more
Citations
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Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus
TL;DR: PMOV-psi- has a defect in the packaging of genomic RNA into virions but can provide in trans the products necessary for virion production, and can be used to produce helper-free stocks of natural or synthetic defective retroviruses.
Journal ArticleDOI
The molecular genetics of cancer
TL;DR: Diverse examples of genetic damage are in hand, and they in turn hint at biochemical explanations for neoplastic growth, suggesting the way may be open to solve the riddles of how normal cells govern their replication and why cancer cells do not.
Journal ArticleDOI
Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA.
TL;DR: The provirus of ATLV integrated in DNA of leukemia T cells from a patient with ATL was molecularly cloned and the complete nucleotide sequence of the proviral genome was determined.
Journal ArticleDOI
The human insulin receptor cDNA: The structural basis for hormone-activated transmembrane signalling
Yousuke Ebina,Leland Ellis,Kurt Jarnagin,Marc Edery,László Gráf,Eric Clauser,Jing-Hsiung Ou,Frank Masiarz,Yuet Wai Kan,Ira D. Goldfine,Richard A. Roth,William J. Rutter +11 more
TL;DR: A cloned approximately 5 kb cDNA (human placenta) contains the coding sequences for the insulin receptor; the nucleotide sequence predicts a 1382 amino acid precursor and the overall structure is reminiscent of the EGF receptor.
Journal ArticleDOI
Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus.
Mark A. Muesing,Douglas H. Smith,C. D. Cabradilla,Charles V. Benton,Laurence A. Lasky,Daniel J. Capon +5 more
TL;DR: The 9,213-nucleotide structure of the AIDS/ lymphadenopathy virus has been determined from molecular clones representing the integrated provirus and viral RNA and the sequence reveals that the virus is highly polymorphic and lacks significant nucleotide homology with type C retroviruses characterized previously.
References
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Journal ArticleDOI
Organization and Expression of Eucaryotic Split Genes Coding for Proteins
R Breathnach,P Chambon +1 more
TL;DR: This paper organizes the organization of protein codes into split genes, a small number of which are expressed in the chickenuct, and discusses generalization, generalization and Molecular Evolution.
Expression of eucaryotic split genes coding for proteins
TL;DR: In this paper, the split genes were described as follows: Globin genes expressed in the chicken o,iduct, Vitellogenin genes, Collagen genes and Actin genes.
Journal ArticleDOI
DNA methylation and gene function
Aharon Razin,Arthur D. Riggs +1 more
TL;DR: There is now reason to believe, from recent studies, that DNA methylation is a key element in the hierarchy of control mechanisms that govern vertebrate gene function and differentiation.
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Chromosomal subunits in active genes have an altered conformation
Harold Weintraub,Mark Groudine +1 more
TL;DR: The results indicate that active genes are probably associated with histones in a subunit conformation in which the associated DNA is particularly sensitive to digestion by deoxyribonuclease I.
Journal ArticleDOI
Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis
TL;DR: The data indicate that, as a rare event, the ALV provirus integrates adjacent to the c-myc gene and that transcription, initiating from a viral promoter, causes enhanced expression of c- myc, leading to neoplastic transformation.