D
Douglas H. Smith
Researcher at Genentech
Publications - 36
Citations - 11182
Douglas H. Smith is an academic researcher from Genentech. The author has contributed to research in topics: Gene & Antigen. The author has an hindex of 26, co-authored 36 publications receiving 11070 citations. Previous affiliations of Douglas H. Smith include Beth Israel Deaconess Medical Center.
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Journal ArticleDOI
Designing CD4 immunoadhesins for AIDS therapy
Daniel J. Capon,Steven M. Chamow,Joyce Mordenti,Scot A. Marsters,Timothy J. Gregory,Hiroaki Mitsuya,Randal A. Byrn,Catherine Lucas,Florian M. Wurm,Jerome E. Groopman,Samuel Broder,Douglas H. Smith +11 more
TL;DR: A newly-constructed antibody-like molecule containing the gp!20-binding domain of the receptor for human immunodeficiency virus blocks HIV-1 infection of T cells and monocytes, making it a good candidate for therapeutic use.
Journal ArticleDOI
Delineation of a region of the human immunodeficiency virus type 1 gp120 glycoprotein critical for interaction with the CD4 receptor
Laurence A. Lasky,Gerald R. Nakamura,Douglas H. Smith,Christopher Fennie,Craig Shimasaki,Eric J. Patzer,Phillip W. Berman,Timothy J. Gregory,Daniel J. Capon +8 more
TL;DR: Using in vitro mutagenesis, it is found that deletion of 12 amino acids from this region of gp120 leads to a complete loss of binding and a single amino acid substitution in this region results in significantly decreased binding, suggesting that sequences within this region are directly involved in the binding of gp 120 to the CD4 receptor.
Journal ArticleDOI
Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors.
TL;DR: Differences among subtypes in the affinities and proportions of such sites suggest the capacity of mAChR subtypes to interact differentially with the cellular effector‐coupling apparatus.
Journal ArticleDOI
Expression of active human factor VIII from recombinant DNA clones
William I. Wood,Daniel J. Capon,Christian C. Simonsen,Dan L. Eaton,Jane Gitschier,Bruce Keyt,Peter H. Seeburg,Douglas H. Smith,P Hollingshead,Karen L. Wion,Eric Delwart,Edward G. D. Tuddenham,Gordon A. Vehar,Richard M. Lawn +13 more
TL;DR: The recombinant protein corrects the clotting time of plasma from haemophiliacs and has many of the biochemical and immunological characteristics of serum-derived factor VIII.
Journal ArticleDOI
Antigen–specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs
Larry L. Green,M.C. Hardy,Catherine E. Maynard-Currie,H. Tsuda,Donna M. Louie,Michael Mendez,Hadi Abderrahim,Masato Noguchi,Douglas H. Smith,Y. Zeng,N.E. David,H. Sasai,D. Garza,D.G. Brenner,Joanna F. Hales,R.P. McGuinness,Daniel J. Capon,Sue Klapholz,Aya Jakobovits +18 more
TL;DR: This work describes a strategy for producing human monoclonal antibodies in mice by introducing large segments of the human heavy and κ light chain loci contained on yeast artificial chromosomes into the mouse germline, which should provide insight into the adoptive human antibody response and permit the development of fully human monocolonal antibodies with therapeutic potential.