From Multiplex Serology to Serolomics-A Novel Approach to the Antibody Response against the SARS-CoV-2 Proteome.
Julia Butt,Rajagopal Murugan,Theresa Hippchen,Sylvia Olberg,Monique van Straaten,Hedda Wardemann,Erec Stebbins,Hans-Georg Kräusslich,Ralf Bartenschlager,Ralf Bartenschlager,Hermann Brenner,Hermann Brenner,Vibor Laketa,Ben Schöttker,Barbara Müller,Uta Merle,Tim Waterboer +16 more
TLDR
In this article, a fluorescent-bead-based SARS-CoV-2 multiplex serology assay for detection of antibody responses to the SARS CoV2 proteome was developed.Abstract:
The emerging SARS-CoV-2 pandemic entails an urgent need for specific and sensitive high-throughput serological assays to assess SARS-CoV-2 epidemiology. We, therefore, aimed at developing a fluorescent-bead based SARS-CoV-2 multiplex serology assay for detection of antibody responses to the SARS-CoV-2 proteome. Proteins of the SARS-CoV-2 proteome and protein N of SARS-CoV-1 and common cold Coronaviruses (ccCoVs) were recombinantly expressed in E. coli or HEK293 cells. Assay performance was assessed in a COVID-19 case cohort (n = 48 hospitalized patients from Heidelberg) as well as n = 85 age- and sex-matched pre-pandemic controls from the ESTHER study. Assay validation included comparison with home-made immunofluorescence and commercial enzyme-linked immunosorbent (ELISA) assays. A sensitivity of 100% (95% CI: 86-100%) was achieved in COVID-19 patients 14 days post symptom onset with dual sero-positivity to SARS-CoV-2 N and the receptor-binding domain of the spike protein. The specificity obtained with this algorithm was 100% (95% CI: 96-100%). Antibody responses to ccCoVs N were abundantly high and did not correlate with those to SARS-CoV-2 N. Inclusion of additional SARS-CoV-2 proteins as well as separate assessment of immunoglobulin (Ig) classes M, A, and G allowed for explorative analyses regarding disease progression and course of antibody response. This newly developed SARS-CoV-2 multiplex serology assay achieved high sensitivity and specificity to determine SARS-CoV-2 sero-positivity. Its high throughput ability allows epidemiologic SARS-CoV-2 research in large population-based studies. Inclusion of additional pathogens into the panel as well as separate assessment of Ig isotypes will furthermore allow addressing research questions beyond SARS-CoV-2 sero-prevalence.read more
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Persistent symptoms in adult patients one year after COVID-19: a prospective cohort study.
Jessica Seeßle,Tim Waterboer,Theresa Hippchen,Julia Simon,Marietta Kirchner,Adeline Lim,Barbara Müller,Uta Merle +7 more
TL;DR: In this article, the authors analyzed a cohort of COVID-19 patients prospectively, and found that neurocognitive long COVID symptoms can persist at least for one year after the symptom onset, and reduce life quality significantly.
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Prevalence of SARS-CoV-2 Infection in Children and Their Parents in Southwest Germany.
Burkhard Tönshoff,Barbara Müller,Roland Elling,Hanna Renk,Peter Meissner,Hartmut Hengel,Sven F. Garbade,Meinhard Kieser,Kathrin Jeltsch,Jürgen Grulich-Henn,Julia Euler,Maximilian Stich,Kristine Chobanyan-Jürgens,Kristine Chobanyan-Jürgens,Maria Zernickel,Ales Janda,Lena Mareike Wölfle,Thomas Stamminger,Thomas Iftner,Tina Ganzenmueller,Christian Schmitt,Tessa Görne,Vibor Laketa,Sylvia Olberg,Anna Plaszczyca,Mirko Cortese,Ralf Bartenschlager,Constantin Pape,Roman Remme,Daniela Huzly,Marcus Panning,Sebastian Weigang,Sebastian Giese,Kevin Ciminski,Jakob Ankerhold,Georg Kochs,Martin Schwemmle,Rupert Handgretinger,Charlotte M. Niemeyer,Corinna Engel,Winfried V. Kern,Georg F. Hoffmann,Axel R. Franz,Philipp Henneke,Klaus-Michael Debatin,Hans-Georg Kräusslich +45 more
TL;DR: In this paper, a large-scale, multicenter, cross-sectional investigation (the COVID-19 BaWu study) enrolled children aged 1 to 10 years and a corresponding parent between April 22 and May 15, 2020, in southwest Germany.
Journal ArticleDOI
Single-reaction multi-antigen serological test for comprehensive evaluation of SARS-CoV-2 patients by flow cytometry.
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TL;DR: In this paper, the Spike glycoprotein, its RBD fragment (the main target for neutralizing antibodies), the nucleocapsid protein, and the main cysteine-like protease were detected in a single reaction.
Journal ArticleDOI
Performance of SARS-CoV-2 Antigens in a Multiplex Bead Assay for Integrated Serological Surveillance of Neglected Tropical and Other Diseases
TL;DR: In this paper , a validation of four SARS-CoV-2 antigens (full-length spike protein, two receptor binding domain proteins, and the nucleocapsid protein) on an existing multiplex bead assay (MBA) for enteric diseases, malaria, and vaccine preventable diseases was conducted.
Journal ArticleDOI
Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics
TL;DR: Test the compatibility of serolomics with dried blood spot (DBS)-derived eluates, which allows for the monitoring of patients and epidemiological analyses in resource-poor settings, and concordant classification of SARS-CoV-2 seropositivity.
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