Development and characterization of an indirect ELISA to detect SARS-CoV-2 spike protein-specific antibodies.
Verena Krähling,Sandro Halwe,Cornelius Rohde,Dirk Becker,Susanne Berghöfer,Christine Dahlke,Markus Eickmann,Meryem S. Ercanoglu,Lutz Gieselmann,Astrid Herwig,Alexandra Kupke,Helena Müller,Petra Neubauer-Rädel,Florian Klein,Christian Keller,Stephan Becker +15 more
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TLDR
In this article, a SARS-CoV-2 spike (S1) protein enzyme-linked immunosorbent assay (ELISA) was developed to detect SARS CoV2-specific antibodies.About:
This article is published in Journal of Immunological Methods.The article was published on 2021-03-01 and is currently open access. It has received 23 citations till now. The article focuses on the topics: Seroconversion.read more
Citations
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Journal ArticleDOI
Artificial intelligence-assisted colorimetric lateral flow immunoassay for sensitive and quantitative detection of COVID-19 neutralizing antibody
H. Tong,Chaoyu Cao,Minli You,Shuang Han,Zhe Liu,Ying Xiao,Wanghong He,Chang-lin Liu,Ping Peng,Zhenrui Xue,Yan Gong,Chun-li Yao,Feng Xu +12 more
TL;DR: Wang et al. as discussed by the authors developed an artificial intelligent (AI)-assisted colorimetric polydopamine nanoparticle (PDA)-based lateral flow immunoassay (LFIA) platform for the sensitive and accurate quantification of neutralizing antibodies produced from vaccinations.
Journal ArticleDOI
Intranasal Administration of a Monoclonal Neutralizing Antibody Protects Mice against SARS-CoV-2 Infection.
Sandro Halwe,Alexandra Kupke,Kanika Vanshylla,Falk Liberta,Henning Gruell,Matthias Zehner,Cornelius Rohde,Verena Krähling,Michelle Gellhorn Serra,Christoph Kreer,Michael Klüver,Lucie Sauerhering,Jörg C. Schmidt,Zheng Cai,Fei Han,David Young,Guangwei Yang,Marek Widera,Manuel Koch,Anke Werner,Lennart Kämper,Nico Becker,Michael S Marlow,Markus Eickmann,Sandra Ciesek,Sandra Ciesek,Felix Schiele,Florian Klein,Stephan Becker +28 more
TL;DR: Zhang et al. as discussed by the authors proposed a fully human monoclonal neutralizing antibody that binds the receptor-binding domain of the SARS-CoV-2 spike protein.
Journal ArticleDOI
Novel approaches for rapid detection of COVID-19 during the pandemic: A review.
TL;DR: A review of the new approaches and sensing technologies that work on COVID-19 diagnosis for easy and successful detection of SARS-CoV-2 virus can be found in this article.
Journal ArticleDOI
Analysis of IgG, IgA and IgM antibodies against SARS-CoV-2 spike protein S1 in convalescent and vaccinated patients with the Pfizer-BioNTech and CanSinoBio vaccines.
Edgar Alonso Melgoza-González,Diana Hinojosa-Trujillo,Mónica Reséndiz-Sandoval,Verónica Mata-Haro,Sofía Hernández-Valenzuela,Melissa García-Vega,Marlene Bravo-Parra,Aldo A. Arvizu-Flores,Olivia Valenzuela,Edgar Velázquez,Alan Soto-Gaxiola,Martha B Gómez-Meza,Fernando Pérez-Jacobo,Luis Villela,Luis Villela,Jesus C. Hernández +15 more
TL;DR: In this paper, the authors developed an indirect ELISA test to evaluate S1 antibodies in convalescent and vaccinated individuals, which showed that IgG antibodies against the S1 protein of SARS-CoV-2 were detected up to 42 weeks after the onset of the symptoms, in contrast to IgA and IgM.
Journal ArticleDOI
Latex Microsphere-Based Bicolor Immunochromatography for Qualitative Detection of Neutralizing Antibody against SARS-CoV-2
Zhan-Hang Liang,Tao Peng,Xueshima Jiao,Yang Zhao,Jie Xie,You Jiang,B. Meng,Xiang Fang,Xiaohui Yu,Xinhua Dai +9 more
TL;DR: A direct, rapid, and point-of-care bicolor lateral flow immunoassay (LFIA) was developed for NAb against SARS-CoV-2 detection without sample pretreatment, and which was based on the principle of NAb-mediated blockage of the interaction between RBD and angiotensin-converting enzyme 2.
References
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Virological assessment of hospitalized patients with COVID-2019.
Roman Wölfel,Victor M. Corman,Wolfgang Guggemos,M Seilmaier,Sabine Zange,Marcel A. Müller,Daniela Niemeyer,Terry Jones,Terry Jones,Patrick Vollmar,Camilla Rothe,Michael Hoelscher,Tobias Bleicker,Sebastian Brünink,Julia Schneider,Rosina Ehmann,Katrin Zwirglmaier,Christian Drosten,Clemens M. Wendtner +18 more
TL;DR: Detailed virological analysis of nine cases of coronavirus disease 2019 (COVID-19) provides proof of active replication of the SARS-CoV-2 virus in tissues of the upper respiratory tract.
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COVID-19 infection: Origin, transmission, and characteristics of human coronaviruses
Muhammad Adnan Shereen,Muhammad Adnan Shereen,Suliman Khan,Abeer Kazmi,Nadia Bashir,Rabeea Siddique +5 more
TL;DR: The approaches for developing effective vaccines and therapeutic combinations to cope with this viral outbreak are discussed and the emergence and pathogenicity of COVID-19 infection and previous human coronaviruses severe acute respiratory syndrome coronavirus (SARS-CoV) and middle east respiratory virus (MERS- coV) is analyzed.
Journal ArticleDOI
Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19)
Li Guo,Lili Ren,Siyuan Yang,Meng Xiao,De Chang,Fan Yang,Charles S. Dela Cruz,Yingying Wang,Chao Wu,Yan Xiao,Lulu Zhang,Lianlian Han,Shengyuan Dang,Yan Xu,Qi Wen Yang,Sheng Yong Xu,Hua Dong Zhu,Yingchun Xu,Qi Jin,Lokesh Sharma,Linghang Wang,Jianwei Wang +21 more
TL;DR: The humoral response to SARS-CoV-2 can aid in the diagnosis of COVID-19, including subclinical cases, and the detection efficiency by IgM ELISA is higher than that of qPCR after 5.5 days of symptom onset.
Journal ArticleDOI
Severe Acute Respiratory Syndrome Coronavirus 2-Specific Antibody Responses in Coronavirus Disease Patients
Nisreen M.A. Okba,Marcel A. Müller,Wentao Li,Chunyan Wang,Corine H. GeurtsvanKessel,Victor M. Corman,Mart M. Lamers,Reina S. Sikkema,Erwin de Bruin,Felicity D. Chandler,Yazdan Yazdanpanah,Quentin Le Hingrat,Diane Descamps,Nadhira Houhou-Fidouh,Chantal B.E.M. Reusken,Berend Jan Bosch,Christian Drosten,Marion Koopmans,Bart L. Haagmans +18 more
TL;DR: It is demonstrated that most PCR-confirmed SARS-CoV-2–infected persons seroconverted by 2 weeks after disease onset, and validated and tested various antigens in different in-house and commercial ELISAs, finding that commercial S1 IgG or IgA ELISA were of lower specificity, and sensitivity varied between the 2 assays; the IgAELISA showed higher sensitivity.
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IgA dominates the early neutralizing antibody response to SARS-CoV-2.
Delphine Sterlin,Delphine Sterlin,Alexis Mathian,Makoto Miyara,Audrey Mohr,François Anna,Laetitia Claër,Paul Quentric,Jehane Fadlallah,Hervé Devilliers,Pascale Ghillani,Cary Gunn,Rick Hockett,Sasi Mudumba,Amélie Guihot,Charles Edouard Luyt,Julien Mayaux,Alexandra Beurton,Salma Fourati,Timothée Bruel,Olivier Schwartz,Jean Marc Lacorte,Hans Yssel,Christophe Parizot,Karim Dorgham,Pierre Charneau,Zahir Amoura,Guy Gorochov +27 more
TL;DR: Specific IgA serum concentrations decreased notably 1 month after the onset of symptoms, but neutralizing IgA remained detectable in saliva for a longer time (days 49 to 73 post-symptoms).
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