Gel Electrophoresis of Avian Leukosis and Sarcoma Viral RNA in Formamide: Comparison with Other Viral and Cellular RNA Species
Peter H. Duesberg,Peter K. Vogt +1 more
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TLDR
Class a RNA was found to have a lower electrophoretic mobility and hence probably a higher molecular weight than class b RNA, and the possible biological significance of this difference is discussed.Abstract:
Class a and class b 30 to 40S RNA subunits obtained by heat dissociation from the 60 to 70S RNA complex of avian tumor viruses were compared with several RNA standards by electrophoresis in formamide-polyacrylamide gels. Class a RNA was found to have a lower electrophoretic mobility and hence probably a higher molecular weight than class b RNA. The absolute molecular weight of class a and b RNA could not be determined with accuracy, because the relationship between logarithm of molecular weight and mobility of the RNA standards was not linear. The size of class a RNA fell into the range of 2.4 × 106 to 3.4 × 106 daltons and that of class b into the range of 2.2 × 106 to 2.9 × 106 daltons, depending on the standards used. The possible biological significance of this difference is discussed.read more
Citations
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Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids
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TL;DR: A simple and sensitive method for detecting, sizing and mapping RNA transcripts from viral or cloned DNAs has been developed and used to examine the cytoplasmic transcripts produced during the early phase of adenovirus 2 (Ad2) infection of HeLa cells.
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DNA related to the transforming gene(s) of avian sarcoma viruses is present in normal avian DNA
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Spliced segments at the 5' terminus of adenovirus 2 late mRNA
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Cell Transformation by RNA Tumor Viruses
TL;DR: The establishment of an assay system for Rous sarcoma virus (RSV) in tissue culture cells (Temin and Rubin, 1958) eventually led to an era of quantitative studies on the mechanism of cellular alteration by viruses.
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Messenger RNA in heat-shocked Drosophila cells.
TL;DR: A complex relationship has been found to exist between the chromosomal sites which form heat shock puffs and the temperature-induced mRNAs, and the finding that at least 25 euchromatic chromosomal Sites which do not form visible heatshock puffs also hybridize in situ to some degree with RNA labeled in heat-shocked cells are discussed.
References
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Journal ArticleDOI
Resolution of Multiple Ribonucleic Acid Species by Polyacrylamide Gel Electrophoresis
Journal ArticleDOI
Differences between the Ribonucleic Acids of Transforming and Nontransforming Avian Tumor Viruses
Peter H. Duesberg,Peter K. Vogt +1 more
TL;DR: The 60–70S RNAs of several transforming and nontransforming avian tumor viruses have different electrophoretic mobilities and it is suggested that the presence of the class a subunit is related to the transforming ability for fibroblasts of the virus.
Journal ArticleDOI
Replication of influenza virus in a continuous cell line: High yield of infective virus from cells inoculated at high multiplicity
Journal ArticleDOI
Dependence of the sedimentation coefficient on molecular weight of RNA after reaction with formaldehyde.
TL;DR: Reaction with formaldehyde has been used to denature the bihelical regions of RNA and convert the polynucleotides into random coils characterized by a single equation relating the logarithm of the sedimentation coefficient to that of the molecular weight.
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