Generation of superoxide anion and chemiluminescence by human monocytes during phagocytosis and on contact with surface-bound immunoglobulin G.
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Oxygen metabolites released from mononuclear phagocytes are highly reactive and could play a part in both the beneficial and detrimental aspects of chronic inflammation.Abstract:
Extent of O-2-release and chemiluminescence, attributed to singlet oxygen, has been compared in human monocytes and neutrophils during phagocytosis, stimulation by the surface-active agent phorbol myristate acetate, or contact with aggregated IgG in a model of immune complex disease. Monocytes generated O-2-and chemiluminescence with each of the three stimuli, although values were significantly less than those of neutrophils from the same individuals. Lymphocytes had no significant activity in either assay with any stimulus. Oxygen metabolites released from mononuclear phagocytes are highly reactive and could play a part in both the beneficial and detrimental aspects of chronic inflammation.read more
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Extracellular cytolysis by activated macrophages and granulocytes. II. Hydrogen peroxide as a mediator of cytotoxicity.
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References
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Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent.
TL;DR: O(2) (-) is made by leukocytes under circumstances which suggest that it may be involved in bacterial killing, and is identified as the agent responsible for the leukocyte-mediated reduction of cytochrome c.
Book
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Evidence for the generation of an electronic excitation state(s) in human polymorphonuclear leukocytes and its participation in bactericidal activity
TL;DR: It is proposed that the CL of PMN reflects the generation of singlet oxygen, 1 0 2 , which acts per , se , as the bactericidal and/or CL species.
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The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes.
Richard B. Johnston,Bernard B. Keele,Hara P. Misra,J E Lehmeyer,L S Webb,R L Baehner,K.V. Rajagopalan +6 more
TL;DR: It would seem clear from these and other studies that the granulo cyte elaborates O2- as a concomitant of the respiratory burst that occurs with phagocytosis, and a requirement for .OH in thephagocytic bactericidal event might explain the apparent requirement for both O1- and H2O2 for such activity.
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Antimicrobial mechanisms in neutrophilic polymorphonuclear leukocytes.
TL;DR: Particular organisms are susceptible to more than one antimicrobial system and thus may be effectively handled by back-up systems when one is absent, and an organism normally killed by the peroxidase system may be handled less efficiently but adequately when MPO is absent by other oxygen-dependent antimicrobial systems.