Germline transmission of donor haplotype following spermatogonial transplantation.
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TLDR
It is reported here that testis-derived cells transplanted into the testis of an infertile mouse will colonize seminiferous tubules and initiate spermatogenesis in > 70% of recipients and with recipients that maintained endogenous sperMatogenesis, testis cell transplantation yielded mice in which up to 80% of progeny were sired by donor-derived spermatozoa.Abstract:
Spermatogenesis is a complex, highly organized, very efficient process that is based upon the capacity of stem cell spermatogonia simultaneously to undergo self-renewal and to provide progeny that differentiate into mature spermatozoa. We report here that testis-derived cells transplanted into the testis of an infertile mouse will colonize seminiferous tubules and initiate spermatogenesis in > 70% of recipients. Testis-derived cells from newborn mice were less effective in colonizing recipient testes than cells from 5- to 15- or 21- to 28-day-old mice. Increasing the number of Sertoli cells in the donor cell population did not increase the efficiency of colonization. Unmodified embryonic stem cells were not able to substitute for testis-derived cells in colonizing testes but instead formed tumors in syngeneic as well as nonsyngeneic hosts. Finally, with recipients that maintained endogenous spermatogenesis, testis cell transplantation yielded mice in which up to 80% of progeny were sired by donor-derived spermatozoa. The technique of spermatogonial cell transplantation should provide a means to generate germline modifications in a variety of species following development of spermatogonial culture techniques and should have additional applications in biology, medicine, and agriculture.read more
Citations
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Journal ArticleDOI
Male reproductive health and environmental xenoestrogens
Jorma Toppari,John Chr. Larsen,Peter Christiansen,Aleksander Giwercman,Philippe Grandjean,Louis J. Guillette,Bernard Jégou,Tina Kold Jensen,Pierre Jouannet,Niels Keiding,Henrik Leffers,John A. McLachlan,Otto A. Meyer,Jørn Müller,E. Rajpert-De Meyts,Thomas H. Scheike,Richard M. Sharpe,John P. Sumpter,Niels E. Skakkebæk +18 more
TL;DR: The growing number of reports demonstrating that common environmental contaminants and natural factors possess estrogenic activity presents the working hypothesis that the adverse trends in male reproductive health may be, at least in part, associated with exposure to estrogenic or other hormonally active environmental chemicals during fetal and childhood development.
Journal ArticleDOI
Spermatogenesis following male germ-cell transplantation
TL;DR: It is reported here that stem cells isolated from testes of donor male mice will repopulate sterile testes when injected into seminiferous tubules and may prove useful as a tool for biomedical science and biotechnology.
Journal ArticleDOI
Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells
Mito Kanatsu-Shinohara,Narumi Ogonuki,Kimiko Inoue,Hiromi Miki,Atsuo Ogura,Shinya Toyokuni,Takashi Shinohara +6 more
TL;DR: In vitro culture of spermatogonial stem cells that proliferate for long periods of time are reported, and gonocytes isolated from neonatal mouse testis proliferated over a 5-month period and restored fertility to congenitally infertile recipient mice following transplantation into seminiferous tubules.
Journal ArticleDOI
Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells
TL;DR: The identification of exogenous factors that allow continuous proliferation of SSCs in vitro establishes the foundation to study the basic biology of S SCs and makes possible germ-line modification by sophisticated technologies.
Journal ArticleDOI
Essential role of Plzf in maintenance of spermatogonial stem cells
Jose A. Costoya,Robin M. Hobbs,Maria Barna,Giorgio Cattoretti,Katia Manova,Meena Sukhwani,Kyle E. Orwig,Debra J. Wolgemuth,Pier Paolo Pandolfi +8 more
TL;DR: Results identify Plzf as a spermatogonia-specific transcription factor in the testis that is required to regulate self-renewal and maintenance of the stem cell pool.
References
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