Patent
Glycolytic promoters for regulated protein expression: protease inhibitor
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TLDR
In this article, yeast promoters associated with expression of specific enzymes in the glycolytic pathway are used for expression of alien DNA, particularly yeast promoters known to provide high enzyme levels of enzymes in GCL, for expressing a mammalian protein, such as alpha-1-antitrypsin.Abstract:
Promoters associated with expression of specific enzymes in the glycolytic pathway are used for expression of alien DNA, particularly yeast promoters known to provide high enzyme levels of enzymes in the glycolytic pathway are employed for expressing a mammalian protein, such as alpha-1-antitrypsin. The promoters include promoters involved in expression of pyruvate kinase, triose phosphate isomerase, phosphoglucose isomerase, phosphoglycerate mutase, hexokinase 1, hexokinase 2, glucokinase, phosphofructose kinase, and aldolase, as well as the glycolytic regulation gene. Particularly, the glycolytic regulation gene can be used in conjunction with promoters in the glycolytic pathway for regulated production of desired proteins.read more
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References
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Journal ArticleDOI
Synthesis and assembly of hepatitis B virus surface antigen particles in yeast
Pablo Valenzuela,Pablo Valenzuela,Angelica Medina,William J. Rutter,Gustav Ammerer,Benjamin D. Hall +5 more
TL;DR: The surface antigen of hepatitis B virus has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences.
Journal ArticleDOI
Cloning and sequence of cDNA coding for alpha 1-antitrypsin.
Kotoku Kurachi,T. Chandra,Sandra J. Friezner Degen,Thomas Taylor White,Thomas L. Marchioro,Savio L. C. Woo,Earl W. Davie +6 more
TL;DR: Comparison of the amino acid sequences of baboon alpha 1-antitrypsin, human antithrombin III, and chicken ovalbumin indicated that these three proteins are about 230% homologous.
Journal ArticleDOI
Sequence homology and structural comparison between the chromosomal human alpha 1-antitrypsin and chicken ovalbumin genes.
Margaret Leicht,George L. Long,T. Chandra,Kotoku Kurachi,Vincent J. Kidd,Myles L. Mace,Earl W. Davie,Savio L. C. Woo +7 more
TL;DR: Human α1-antitrypsin and chicken ovalbumin show significant sequence homology and belong to a common protein super-family, yet the number, position and size of intervening sequences reveal that the two genes are dissimilar.
Journal Article
Nucleotide sequence of the triose phosphate isomerase gene of Saccharomyces cerevisiae.
Alber T,Kawasaki G +1 more
TL;DR: The gene coding for the glycolytic enzyme triose phosphate isomerase (TPI1) was isolated from a yeast library in the shuttle vector pYE13 by selecting for a deletion mutant of the plasmid which enhances expression of the otherwise dormant yeast gene in E. coli.